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Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis

Author

Listed:
  • Adrian T. Saurin

    (Molecular Cancer Research and Cancer Genomics Centre, University Medical Center Utrecht)

  • Maike S. van der Waal

    (University Medical Center Utrecht)

  • René H. Medema

    (University Medical Center Utrecht)

  • Susanne M.A. Lens

    (University Medical Center Utrecht)

  • Geert J.P.L. Kops

    (Molecular Cancer Research and Cancer Genomics Centre, University Medical Center Utrecht)

Abstract

The mitotic checkpoint prevents mitotic exit until all chromosomes are attached to spindle microtubules. Aurora B kinase indirectly invokes this checkpoint by destabilizing incorrect attachments; however, a more direct role remains controversial. In contrast, activity of the kinase Mps1 is indispensible for the mitotic checkpoint. Here we show that Aurora B and Hec1 are needed for efficient Mps1 recruitment to unattached kinetochores, allowing rapid Mps1 activation at the onset of mitosis. Live monitoring of cyclin B degradation reveals that this is essential to establish the mitotic checkpoint quickly at the start of mitosis. Delayed Mps1 activation and checkpoint establishment upon Aurora B inhibition or Hec1 depletion are rescued by tethering Mps1 to kinetochores, demonstrating that Mps1 recruitment is the primary role of Aurora B and Hec1 in mitotic checkpoint signalling. These data demonstrate a direct role for Aurora B in initiating the mitotic checkpoint rapidly at the onset of mitosis.

Suggested Citation

  • Adrian T. Saurin & Maike S. van der Waal & René H. Medema & Susanne M.A. Lens & Geert J.P.L. Kops, 2011. "Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis," Nature Communications, Nature, vol. 2(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1319
    DOI: 10.1038/ncomms1319
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