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Intestinal epithelial stem cells do not protect their genome by asymmetric chromosome segregation

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Listed:
  • Marion Escobar

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203)

  • Pierre Nicolas

    (INRA, UR-1077, Mathématique Informatique et Génome)

  • Fatiha Sangar

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203
    Present addresses: INSERM, UMR-S938-UPMC, Centre de Recherche Saint-Antoine, F-75000 Paris, France (F.S.); Université de Montpellier 2, Plateforme qPCR Haut débit Montpellier GenomiX, cc91, F-34000 Montpellier, France (P.C.).)

  • Sabine Laurent-Chabalier

    (CNRS, UPR-1142, Institut de Génétique Humaine)

  • Philippe Clair

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203
    Present addresses: INSERM, UMR-S938-UPMC, Centre de Recherche Saint-Antoine, F-75000 Paris, France (F.S.); Université de Montpellier 2, Plateforme qPCR Haut débit Montpellier GenomiX, cc91, F-34000 Montpellier, France (P.C.).)

  • Dominique Joubert

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203)

  • Philippe Jay

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203)

  • Catherine Legraverend

    (CNRS, UMR-5203, Institut de Génomique Fonctionnelle
    INSERM, U661
    Universités de Montpellier 1&2, UMR-5203)

Abstract

The idea that stem cells of adult tissues with high turnover are protected from DNA replication-induced mutations by maintaining the same 'immortal' template DNA strands together through successive divisions has been tested in several tissues. In the epithelium of the small intestine, the provided evidence was based on the assumption that stem cells are located above Paneth cells. The results of genetic lineage-tracing experiments point instead to crypt base columnar cells intercalated between Paneth cells as bona fide stem cells. Here we show that these cells segregate most, if not all, of their chromosomes randomly, both in the intact and in the regenerating epithelium. Therefore, the 'immortal' template DNA strand hypothesis does not apply to intestinal epithelial stem cells, which must rely on other strategies to avoid accumulating mutations.

Suggested Citation

  • Marion Escobar & Pierre Nicolas & Fatiha Sangar & Sabine Laurent-Chabalier & Philippe Clair & Dominique Joubert & Philippe Jay & Catherine Legraverend, 2011. "Intestinal epithelial stem cells do not protect their genome by asymmetric chromosome segregation," Nature Communications, Nature, vol. 2(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1260
    DOI: 10.1038/ncomms1260
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