IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v2y2011i1d10.1038_ncomms1157.html
   My bibliography  Save this article

Synthetic human cell fate regulation by protein-driven RNA switches

Author

Listed:
  • Hirohide Saito

    (Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku
    International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku
    The Hakubi Center, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku)

  • Yoshihiko Fujita

    (Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku
    International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku)

  • Shunnichi Kashida

    (Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku)

  • Karin Hayashi

    (International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku)

  • Tan Inoue

    (Laboratory of Gene Biodynamics, Graduate School of Biostudies, Kyoto University, Oiwake-cho, Kitashirakawa, Sakyo-ku
    International Cooperative Research Project, Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku)

Abstract

Understanding how to control cell fate is crucial in biology, medical science and engineering. In this study, we introduce a method that uses an intracellular protein as a trigger for regulating human cell fate. The ON/OFF translational switches, composed of an intracellular protein L7Ae and its binding RNA motif, regulate the expression of a desired target protein and control two distinct apoptosis pathways in target human cells. Combined use of the switches demonstrates that a specific protein can simultaneously repress and activate the translation of two different mRNAs: one protein achieves both up- and downregulation of two different proteins/pathways. A genome-encoded protein fused to L7Ae controlled apoptosis in both directions (death or survival) depending on its cellular expression. The method has potential for curing cellular defects or improving the intracellular production of useful molecules by bypassing or rewiring intrinsic signal networks.

Suggested Citation

  • Hirohide Saito & Yoshihiko Fujita & Shunnichi Kashida & Karin Hayashi & Tan Inoue, 2011. "Synthetic human cell fate regulation by protein-driven RNA switches," Nature Communications, Nature, vol. 2(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1157
    DOI: 10.1038/ncomms1157
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms1157
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms1157?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1157. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.