IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v1y2010i1d10.1038_ncomms1145.html
   My bibliography  Save this article

Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes

Author

Listed:
  • Oren Kobiler

    (Princeton University)

  • Yaron Lipman

    (The Program in Applied and Computational Mathematics, Princeton University)

  • Kate Therkelsen

    (The Program in Applied and Computational Mathematics, Princeton University)

  • Ingrid Daubechies

    (The Program in Applied and Computational Mathematics, Princeton University)

  • Lynn W. Enquist

    (Princeton University)

Abstract

Whether all the infectious herpesvirus particles entering a cell are able to replicate and/or express their genomes is not known. Here, we developed a general method to determine the number of viral genomes expressed in an infected cell. We constructed and analysed fluorophore expression from a recombinant pseudorabies virus (PRV263) carrying a Brainbow cassette (Cre-conditional expression of different fluorophores). Using three isogenic strains derived from PRV263, each expressing a single fluorophore, we analysed the colour composition of cells infected with these three viruses at different multiplicities. We estimate that fewer than seven incoming genomes are expressed per cell. In addition, those templates that are expressed are the genomes selected for replication and packaging into virions. This finite limit on the number of viral genomes that can be expressed is an intrinsic property of the infected cell and may be influenced by viral and cellular factors.

Suggested Citation

  • Oren Kobiler & Yaron Lipman & Kate Therkelsen & Ingrid Daubechies & Lynn W. Enquist, 2010. "Herpesviruses carrying a Brainbow cassette reveal replication and expression of limited numbers of incoming genomes," Nature Communications, Nature, vol. 1(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1145
    DOI: 10.1038/ncomms1145
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms1145
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms1145?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1145. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.