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Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis

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  • Andreas Otto

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

  • Jörg Bernhardt

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

  • Hanna Meyer

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute of Pharmacy)

  • Marc Schaffer

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

  • Florian-A. Herbst

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

  • Juliane Siebourg

    (ETH Zürich, Mattenstrasse 26)

  • Ulrike Mäder

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology
    Ernst-Moritz-Arndt-Universität Greifswald, Interfaculty Institute for Genetics and Functional Genomics)

  • Michael Lalk

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute of Pharmacy)

  • Michael Hecker

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

  • Dörte Becher

    (Ernst-Moritz-Arndt-Universität Greifswald, Institute for Microbiology)

Abstract

Functional genomics of the Gram-positive model organism Bacillus subtilis reveals valuable insights into basic concepts of cell physiology. In this study, we monitor temporal changes in the proteome, transcriptome and extracellular metabolome of B. subtilis caused by glucose starvation. For proteomic profiling, a combination of in vivo metabolic labelling and shotgun mass spectrometric analysis was carried out for five different proteomic subfractions (cytosolic, integral membrane, membrane, surface and extracellular proteome fraction), leading to the identification of ∼52% of the predicted proteome of B. subtilis. Quantitative proteomic and corresponding transcriptomic data were analysed with Voronoi treemaps linking functional classification and relative expression changes of gene products according to their fate in the stationary phase. The obtained data comprise the first comprehensive profiling of changes in the membrane subfraction and allow in-depth analysis of major physiological processes, including monitoring of protein degradation.

Suggested Citation

  • Andreas Otto & Jörg Bernhardt & Hanna Meyer & Marc Schaffer & Florian-A. Herbst & Juliane Siebourg & Ulrike Mäder & Michael Lalk & Michael Hecker & Dörte Becher, 2010. "Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis," Nature Communications, Nature, vol. 1(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:1:y:2010:i:1:d:10.1038_ncomms1137
    DOI: 10.1038/ncomms1137
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