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Structural insights into the secretin complex of a type IVb pilus system

Author

Listed:
  • Chenchen Pei

    (Hunan University)

  • Hui Sun

    (Hunan University)

  • Yinliang Qi

    (Hefei Hospital Affiliated to Anhui Medical University)

  • Xiaomei Li

    (Shanxi Academy of Advanced Research and Innovation)

  • Zhixiong Fang

    (Hunan University)

  • Manjuan Tang

    (Hunan University)

  • Rujing Zhang

    (Hunan University)

  • Zhaofeng Yan

    (Hunan University)

  • Meng Yin

    (Hunan University)

Abstract

The bundle-forming pilus (BFP) system in enteropathogenic Escherichia coli (EPEC) produces type IVb pili that enable bacterial auto-aggregation, facilitating bacterial adhesion, colonization, and virulence. One of its components, lipoprotein BfpB, interacts with BfpG to form a secretin channel complex that enables pilus translocation across the outer membrane. Here, we report a high-resolution cryo-EM structure of the BfpB-BfpG complex, revealing a 17:17 stoichiometry with stable zigzag-like interactions between BfpG and BfpB near the N3 ring. Secretin BfpB consists of three β-barrels, including an additional N3 barrel that is crucial for BFP biogenesis. As a lipoprotein-type secretin, BfpB possesses an N-terminal LG domain that bridges the N0 domain and the outer membrane, ensuring its correct localization to the bacterial outer membrane. The C-terminal region of the LG domain mediates binding to BfpG, and disruption of these interactions impairs BFP biogenesis. Our results advance our understanding of the assembly mechanism of secretin complexes within the secretin superfamily.

Suggested Citation

  • Chenchen Pei & Hui Sun & Yinliang Qi & Xiaomei Li & Zhixiong Fang & Manjuan Tang & Rujing Zhang & Zhaofeng Yan & Meng Yin, 2025. "Structural insights into the secretin complex of a type IVb pilus system," Nature Communications, Nature, vol. 16(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-65063-w
    DOI: 10.1038/s41467-025-65063-w
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    1. Sean Miletic & Dirk Fahrenkamp & Nikolaus Goessweiner-Mohr & Jiri Wald & Maurice Pantel & Oliver Vesper & Vadim Kotov & Thomas C. Marlovits, 2021. "Substrate-engaged type III secretion system structures reveal gating mechanism for unfolded protein translocation," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    2. Alexander Neuhaus & Muniyandi Selvaraj & Ralf Salzer & Julian D. Langer & Kerstin Kruse & Lennart Kirchner & Kelly Sanders & Bertram Daum & Beate Averhoff & Vicki A. M. Gold, 2020. "Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    3. Sara J. Weaver & Davi R. Ortega & Matthew H. Sazinsky & Triana N. Dalia & Ankur B. Dalia & Grant J. Jensen, 2020. "Publisher Correction: CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholera," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    4. Josh Abramson & Jonas Adler & Jack Dunger & Richard Evans & Tim Green & Alexander Pritzel & Olaf Ronneberger & Lindsay Willmore & Andrew J. Ballard & Joshua Bambrick & Sebastian W. Bodenstein & David , 2024. "Addendum: Accurate structure prediction of biomolecular interactions with AlphaFold 3," Nature, Nature, vol. 636(8042), pages 4-4, December.
    5. Anastasia A. Chernyatina & Harry H. Low, 2019. "Core architecture of a bacterial type II secretion system," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    6. Rebecca Conners & Mathew McLaren & Urszula Łapińska & Kelly Sanders & M. Rhia L. Stone & Mark A. T. Blaskovich & Stefano Pagliara & Bertram Daum & Jasna Rakonjac & Vicki A. M. Gold, 2021. "CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    7. Junfeng Liu & Gunnar N. Eastep & Virginija Cvirkaite-Krupovic & Shane T. Rich-New & Mark A. B. Kreutzberger & Edward H. Egelman & Mart Krupovic & Fengbin Wang, 2024. "Two distinct archaeal type IV pili structures formed by proteins with identical sequence," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    8. Josh Abramson & Jonas Adler & Jack Dunger & Richard Evans & Tim Green & Alexander Pritzel & Olaf Ronneberger & Lindsay Willmore & Andrew J. Ballard & Joshua Bambrick & Sebastian W. Bodenstein & David , 2024. "Accurate structure prediction of biomolecular interactions with AlphaFold 3," Nature, Nature, vol. 630(8016), pages 493-500, June.
    9. J. Hu & L. J. Worrall & C. Hong & M. Vuckovic & C. E. Atkinson & N. Caveney & Z. Yu & N. C. J. Strynadka, 2018. "Cryo-EM analysis of the T3S injectisome reveals the structure of the needle and open secretin," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    10. Subramania Kolappan & Mathieu Coureuil & Xiong Yu & Xavier Nassif & Edward H. Egelman & Lisa Craig, 2016. "Structure of the Neisseria meningitidis Type IV pilus," Nature Communications, Nature, vol. 7(1), pages 1-12, December.
    11. Sara J. Weaver & Davi R. Ortega & Matthew H. Sazinsky & Triana N. Dalia & Ankur B. Dalia & Grant J. Jensen, 2020. "CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    12. Matteo Tassinari & Marta Rudzite & Alain Filloux & Harry H. Low, 2023. "Assembly mechanism of a Tad secretion system secretin-pilotin complex," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    13. L. J. Worrall & C. Hong & M. Vuckovic & W. Deng & J. R. C. Bergeron & D. D Majewski & R. K. Huang & T. Spreter & B. B. Finlay & Z. Yu & N. C. J. Strynadka, 2016. "Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body," Nature, Nature, vol. 540(7634), pages 597-601, December.
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