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HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane

Author

Listed:
  • Tashi Tsering

    (University of Fribourg, Department of Biology)

  • Martin Donato

    (University of Fribourg, Department of Biology)

  • Despina Samakovli

    (Agricultural University of Athens, School of Applied Biology and Biotechnology, Department of Biotechnology)

  • Dimitra Milioni

    (Agricultural University of Athens, School of Applied Biology and Biotechnology, Department of Biotechnology)

  • Francesca Romana Iacobini

    (University of Fribourg, Department of Biology)

  • Konstantinos Panagiotopoulos

    (Agricultural University of Athens, School of Applied Biology and Biotechnology, Department of Biotechnology)

  • Panagiota-Konstantinia Plitsi

    (Agricultural University of Athens, School of Applied Biology and Biotechnology, Department of Biotechnology)

  • Elisa Azarello

    (Università di Firenze)

  • Stefano Mancuso

    (Università di Firenze)

  • Vendula Pukyšová

    (Mendel Centre for Plant Genomics and Proteomics Masaryk University, CEITEC MU)

  • Marta Zwiewka

    (Mendel Centre for Plant Genomics and Proteomics Masaryk University, CEITEC MU)

  • Tomasz Nodzynski

    (Mendel Centre for Plant Genomics and Proteomics Masaryk University, CEITEC MU)

  • Michael Stumpe

    (University of Fribourg, Department of Biology)

  • Jutta Ludwig-Müller

    (Technische Universität Dresden, Faculty of Biology)

  • Aurélien Bailly

    (University of Zürich)

  • Polydefkis Hatzopoulos

    (Agricultural University of Athens, School of Applied Biology and Biotechnology, Department of Biotechnology)

  • Markus M. Geisler

    (University of Fribourg, Department of Biology)

Abstract

Closely related FKBP orthologs, FKBP42/TWISTED DWARF1 (TWD1) and FKBP38, have been shown to control the biogenesis of plant and mammalian ATP-binding cassette (ABC) transporters, respectively. However, the mechanistic role of the described FKBP-ABCB interaction is widely unknown. Here, we verify cytosolic HEAT-SHOCK PROTEIN90 (HSP90) isoforms as valid interactors of TWD1 and map HSP90 binding to an amphiphilic alpha-helix preceding its TPR domain. We provide pharmacological and genetic evidence that a subset of TWD1-interacting ABCBs, in contrast to mammalian ABCBs, are constitutive HSP90 clients in plants. This effect and its specificity are presumably provided by TWD1. Our data strongly correlate the impact of HSP90 inhibition on ABCB-mediated development and ABCB plasma membrane stability on the one hand and ABCB cycling rate on the other. In summary, we uncover a dynamic mechanism of HSP90 for differential stabilization of the plasma membrane ABCB isoforms to regulate polar auxin transport and to confer developmental plasticity.

Suggested Citation

  • Tashi Tsering & Martin Donato & Despina Samakovli & Dimitra Milioni & Francesca Romana Iacobini & Konstantinos Panagiotopoulos & Panagiota-Konstantinia Plitsi & Elisa Azarello & Stefano Mancuso & Vend, 2025. "HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63780-w
    DOI: 10.1038/s41467-025-63780-w
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    References listed on IDEAS

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    1. Géraldine Brunoud & Darren M. Wells & Marina Oliva & Antoine Larrieu & Vincent Mirabet & Amy H. Burrow & Tom Beeckman & Stefan Kepinski & Jan Traas & Malcolm J. Bennett & Teva Vernoux, 2012. "A novel sensor to map auxin response and distribution at high spatio-temporal resolution," Nature, Nature, vol. 482(7383), pages 103-106, February.
    2. Yuqin Zhang & Victoria Nasser & Odelia Pisanty & Moutasem Omary & Nikolai Wulff & Martin Donato & Iris Tal & Felix Hauser & Pengchao Hao & Ohad Roth & Hillel Fromm & Julian I. Schroeder & Markus Geisl, 2018. "A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    3. Niko Geldner & Jiří Friml & York-Dieter Stierhof & Gerd Jürgens & Klaus Palme, 2001. "Auxin transport inhibitors block PIN1 cycling and vesicle trafficking," Nature, Nature, vol. 413(6854), pages 425-428, September.
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