IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-63421-2.html
   My bibliography  Save this article

Injectable therapeutic system incorporating neurogenesis-programmed stem cells concomitantly promoting muscle regeneration treats stress urinary incontinence

Author

Listed:
  • Wenzhuo Fang

    (Shanghai Jiao Tong University)

  • Xuan Du

    (Shanghai Jiao Tong University)

  • Ranxing Yang

    (Shanghai Jiao Tong University)

  • Meng Liu

    (Shanghai Jiao Tong University)

  • Ming Yang

    (Shanghai Jiao Tong University)

  • Yangwang Jin

    (Shanghai Jiao Tong University)

  • Guo Gao

    (Shanghai Jiao Tong University)

  • Qiang Fu

    (Shanghai Jiao Tong University)

  • Ying Wang

    (Shanghai Jiao Tong University)

Abstract

Stress urinary incontinence (SUI) remains a significant clinical challenge due to the lack of strategies that simultaneously address muscle degeneration, neurogenic atrophy, and vascular deficits. Here, we report an innovative injectable system that combines a thermo-responsive poly(N-isopropylacrylamide)-COOH/leucine/decellularized extracellular matrix hydrogel with adipose-derived stem cells (ADSCs) pre-programmed by zeolitic imidazolate framework-8/polyethylene glycol 200@magnesium (ZIF-8/PEG200@Mg) nanoparticles. In vitro, programmed ADSCs exhibit enhanced neurogenic differentiation, while the hydrogels support robust myogenic activity and cell viability. In a female rat model of SUI—chosen to reflect the higher prevalence of SUI in women—the composite system leads to a marked improvement in leak point pressure (LPP) and restores urethral sphincter function. Mechanistic analyses reveals upregulation of muscle regeneration (e.g., Myoz1, Smyd1) and neurogenesis/neuromuscular junction stabilization (NMJ) stabilization genes (e.g., Dok7, Musk), highlighting a coordinated multi-lineage regenerative process. This work establishes an integrated regeneration-plus-support injectable strategy, offering a regenerative medicine-based approach that surpasses conventional bulking or sling therapies for SUI.

Suggested Citation

  • Wenzhuo Fang & Xuan Du & Ranxing Yang & Meng Liu & Ming Yang & Yangwang Jin & Guo Gao & Qiang Fu & Ying Wang, 2025. "Injectable therapeutic system incorporating neurogenesis-programmed stem cells concomitantly promoting muscle regeneration treats stress urinary incontinence," Nature Communications, Nature, vol. 16(1), pages 1-21, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63421-2
    DOI: 10.1038/s41467-025-63421-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-63421-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-63421-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Pihu Mehrotra & James Jablonski & John Toftegaard & Yali Zhang & Shahryar Shahini & Jianmin Wang & Carey W. Hung & Reilly Ellis & Gabriella Kayal & Nika Rajabian & Song Liu & Kelly C. S. Roballo & Sus, 2024. "Skeletal muscle reprogramming enhances reinnervation after peripheral nerve injury," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Xin Zhang & Chao Yang & Chengle Zhang & Junqiang Wu & Xiang Zhang & Jiayang Gao & Xuejie Wang & Leung Ting Chan & Yiren Zhou & Yujun Chen & Sindy Sing Ting Tam & Shuhang Chen & Yuqian Ma & Wing-Ho Yun, 2025. "Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement," Nature Communications, Nature, vol. 16(1), pages 1-20, December.
    3. Muriel Sébastien & Alexandra L. Paquette & Emily N. P. Prowse & Adam G. Hendricks & Gary J. Brouhard, 2025. "Doublecortin restricts neuronal branching by regulating tubulin polyglutamylation," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63421-2. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.