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Root-to-shoot mobile mRNA CmoKARI1 promotes JA-Ile biosynthesis to confer chilling tolerance in grafted cucumbers

Author

Listed:
  • Miao Zhang

    (China Agricultural University)

  • Wenqian Liu

    (China Agricultural University)

  • Cuicui Wang

    (China Agricultural University)

  • Shujin Lin

    (Fuzhou University)

  • Ying Chen

    (China Agricultural University)

  • Heran Cui

    (China Agricultural University)

  • Chenggang Xiang

    (Honghe University)

  • Yujia Ma

    (China Agricultural University)

  • Xiaojun Li

    (China Agricultural University)

  • Yongzhen Lu

    (China Agricultural University)

  • Xiao Han

    (Fuzhou University)

  • Yihan Dong

    (Université de Strasbourg
    Institut de Génétique et de Biologie Moléculaire et Cellulaire)

  • Lihong Gao

    (China Agricultural University)

  • Wenna Zhang

    (China Agricultural University)

Abstract

Pumpkin rootstock is commonly used to graft cucurbit crops, improving their ability to withstand stress. While the significance of systemic signals from rootstocks to scions is recognized, the role of root-to-shoot transported mRNAs remains understudied. Cucumber plants often face growth and productivity limitations due to low temperatures. To shed light on the enhancement of chilling tolerance in grafted cucumber scions by pumpkin-derived mRNAs, we revisit the metabolomic and transcriptomic dataset from the cucumber/pumpkin heterograft under chilling condition. We identify pumpkin Ketol-acid reductoisomerase 1 (CmoKARI1) as the key mobile mRNA that specifically travels from pumpkin rootstock to cucumber scion upon early chilling stress. Overexpressing CmoKARI1 results in increased isoleucine level and chilling tolerance in both cucumber and Arabidopsis. The increased isoleucine is further used to synthesize JA-Ile conjugates, activating JA-Ile signaling and enabling heterografts to weather low temperatures. This study represents the instance of a unidirectional mobile mRNA triggered by specific environmental cues.

Suggested Citation

  • Miao Zhang & Wenqian Liu & Cuicui Wang & Shujin Lin & Ying Chen & Heran Cui & Chenggang Xiang & Yujia Ma & Xiaojun Li & Yongzhen Lu & Xiao Han & Yihan Dong & Lihong Gao & Wenna Zhang, 2025. "Root-to-shoot mobile mRNA CmoKARI1 promotes JA-Ile biosynthesis to confer chilling tolerance in grafted cucumbers," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-63228-1
    DOI: 10.1038/s41467-025-63228-1
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    References listed on IDEAS

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    1. A. Chini & S. Fonseca & G. Fernández & B. Adie & J. M. Chico & O. Lorenzo & G. García-Casado & I. López-Vidriero & F. M. Lozano & M. R. Ponce & J. L. Micol & R. Solano, 2007. "The JAZ family of repressors is the missing link in jasmonate signalling," Nature, Nature, vol. 448(7154), pages 666-671, August.
    2. Bryan Thines & Leron Katsir & Maeli Melotto & Yajie Niu & Ajin Mandaokar & Guanghui Liu & Kinya Nomura & Sheng Yang He & Gregg A. Howe & John Browse, 2007. "JAZ repressor proteins are targets of the SCFCOI1 complex during jasmonate signalling," Nature, Nature, vol. 448(7154), pages 661-665, August.
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