IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-62348-y.html
   My bibliography  Save this article

Unimolecular near-infrared chemiluminescent reporter for cascaded multiplex imaging of ischemia-reperfusion injury in the liver-kidney axis

Author

Listed:
  • Bankang Ruan

    (Sun Yat-sen University)

  • Weiliang Deng

    (Sun Yat-sen University)

  • Jingjing Che

    (Sun Yat-sen University)

  • Shujuan Yi

    (Sun Yat-sen University)

  • Jie Liu

    (University of Chinese Academy of Sciences)

  • Weiping Xu

    (University of Chinese Academy of Sciences)

  • Yuyan Jiang

    (Stanford University School of Medicine)

  • Ya zhou

    (Sun Yat-sen University)

  • Pan Xie

    (Army Medical University)

  • Huhai Zhang

    (Army Medical University)

  • Hongwen Zhao

    (Army Medical University)

  • Jiaguo Huang

    (Sun Yat-sen University)

Abstract

Acute kidney injury (AKI) frequently arises as a complication of hepatic ischemia-reperfusion injury (HIRI), yet simultaneous optical imaging of both remains challenging due to the lack of unimolecular dual-responsive probes. Herein, we report a library of hemicyanine-based chemiluminophores (HCLs) with tunable emission to second near-infrared window (725 − 1025 nm) achieved by integrating bicyclic dioxetane onto hemicyanine skeletons to develop multiple-responsive chemiluminescent probes. HCL1 and HCL5 respectively emitting at 725 nm and 1025 nm are selected to construct a cascaded activatable reporter CAR for crosstalk-free duplex chemiluminescence imaging of interlinked biomarkers. Following systemic injection to male mice, CAR preferentially accumulates in the liver and reports HIRI-associated superoxide anion (O2•−), which initiates self-fragmentation and liberates the secondary reporter KIR into kidneys to report AKI-associated N-acetyl-β-D-glucosaminidase (NAG). Such mechanism allows CAR to serve as a reservoir for gradual release of AKI reporters, providing a significantly prolonged imaging window compared to co-administering separate probes. CAR further permits remote detection of HIRI-induced AKI via urinalysis. This study not only offers a powerful tool for simultaneous detection of HIRI and HIRI-induced AKI, but also highlights a unimolecular probe design for ultrasensitive detection of deeply-seated intercorrelated diseases.

Suggested Citation

  • Bankang Ruan & Weiliang Deng & Jingjing Che & Shujuan Yi & Jie Liu & Weiping Xu & Yuyan Jiang & Ya zhou & Pan Xie & Huhai Zhang & Hongwen Zhao & Jiaguo Huang, 2025. "Unimolecular near-infrared chemiluminescent reporter for cascaded multiplex imaging of ischemia-reperfusion injury in the liver-kidney axis," Nature Communications, Nature, vol. 16(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62348-y
    DOI: 10.1038/s41467-025-62348-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-62348-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-62348-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Yue Jiang & Min Zhao & Jia Miao & Wan Chen & Yuan Zhang & Minqian Miao & Li Yang & Qing Li & Qingqing Miao, 2024. "Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Renye Yue & Zhe Li & Huiyi Liu & Youjuan Wang & Yuhang Li & Rui Yin & Baoli Yin & Haisheng Qian & Heemin Kang & Xiaobing Zhang & Guosheng Song, 2024. "Imaging-guided companion diagnostics in radiotherapy by monitoring APE1 activity with afterglow and MRI imaging," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    3. Lingfei Lu & Benhao Li & Suwan Ding & Yong Fan & Shangfeng Wang & Caixia Sun & Mengyao Zhao & Chun-Xia Zhao & Fan Zhang, 2020. "NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Qian Zhang & Bin Song & Yanan Xu & Yunmin Yang & Jian Ji & Wenjun Cao & Jianping Lu & Jiali Ding & Haiting Cao & Binbin Chu & Jiaxu Hong & Houyu Wang & Yao He, 2023. "In vivo bioluminescence imaging of natural bacteria within deep tissues via ATP-binding cassette sugar transporter," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Yuling Xu & Chonglu Li & Shuai Lu & Zhizheng Wang & Shuang Liu & Xiujun Yu & Xiaopeng Li & Yao Sun, 2022. "Construction of emissive ruthenium(II) metallacycle over 1000 nm wavelength for in vivo biomedical applications," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Yongchao Liu & Lili Teng & Yifan Lyu & Guosheng Song & Xiao-Bing Zhang & Weihong Tan, 2022. "Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Yue Jiang & Min Zhao & Jia Miao & Wan Chen & Yuan Zhang & Minqian Miao & Li Yang & Qing Li & Qingqing Miao, 2024. "Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Yufu Tang & Yuanyuan Li & Chunxu He & Zhen Wang & Wei Huang & Quli Fan & Bin Liu, 2025. "NIR-II-excited off-on-off fluorescent nanoprobes for sensitive molecular imaging in vivo," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    6. Lei Ding & Xiaolong Zhang & Peiyuan Wang & Jianmei Ke & Yang Zhou & Ming Wu & Zuwu Wei & Yanbing Cao & Hongsheng Li & Geng Chen & Guangwei Zheng & Yongyi Zeng & Maochun Hong & Xiaolong Liu, 2025. "Granzyme B activated near-infrared-II ratiometric fluorescent nanoprobe for early detection of tumor response to immunotherapy," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    7. Diankai Liu & Zixu He & Wenjie Gao & Jizhen Shang & Yiqing Yang & Xiaofan Zhang & Xiaohua Li & Huimin Ma & Wen Shi, 2025. "Near-infrared II cyanine fluorophores with large stokes shift engineered by regulating respective absorption and emission," Nature Communications, Nature, vol. 16(1), pages 1-10, December.
    8. Dongsheng Tang & Minhui Cui & Bin Wang & Ganghao Liang & Hanchen Zhang & Haihua Xiao, 2024. "Nanoparticles destabilizing the cell membranes triggered by NIR light for cancer imaging and photo-immunotherapy," Nature Communications, Nature, vol. 15(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62348-y. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.