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Multiplex bead assays enable integrated serological surveillance and reveal cross-pathogen vulnerabilities in Zambezia Province, Mozambique

Author

Listed:
  • Andrea C. Carcelen

    (Johns Hopkins Bloomberg School of Public Health)

  • Celso Monjane

    (Instituto Nacional de Saúde)

  • Sophie Bérubé

    (The University of Florida)

  • Saki Takahashi

    (Johns Hopkins Bloomberg School of Public Health)

  • Thebora Sultane

    (Instituto Nacional de Saúde)

  • Imelda Chelene

    (Instituto Nacional de Saúde)

  • Gretchen Cooley

    (Centers for Disease Control and Prevention)

  • E. Brook Goodhew

    (Centers for Disease Control and Prevention)

  • Catriona Patterson

    (London School of Hygiene and Tropical Medicine)

  • Kevin Tetteh

    (London School of Hygiene and Tropical Medicine)

  • Manuel Mutambe

    (Instituto Nacional de Saúde)

  • Melissa M. Higdon

    (Johns Hopkins Bloomberg School of Public Health)

  • George Mwinnyaa

    (Johns Hopkins Bloomberg School of Public Health)

  • Gilberto Nhapure

    (Instituto Nacional de Estatística)

  • Pedro Duce

    (Instituto Nacional de Estatística)

  • Diana L. Martin

    (Centers for Disease Control and Prevention)

  • Christopher Drakeley

    (London School of Hygiene and Tropical Medicine)

  • William J. Moss

    (Johns Hopkins Bloomberg School of Public Health
    Johns Hopkins Bloomberg School of Public Health)

  • Ivalda Macicame

    (Instituto Nacional de Saúde)

Abstract

Multiplex serological assays simultaneously measure antibodies to multiple antigens, furnishing insights into exposure and susceptibility to several pathogens and cross-pathogen vulnerabilities. Our serosurvey tests dried blood spots from 1292 individuals for IgG antibodies to 35 antigens from 18 pathogens using a multiplex bead assay for vaccine preventable diseases, malaria, SARS-CoV-2, neglected tropical diseases, and enteric pathogens in Mozambique. We produce pathogen-specific seroprevalence estimates and age-seroprevalence curves and identify spatial differences in seroprevalence. Rural clusters have higher odds of seropositivity to most NTDs neglected tropical diseases, Plasmodium falciparum malaria, and enteric pathogens, but lower odds of seropositivity to SARS-CoV-2 and vaccine preventable diseases compared to urban clusters. This co-occurrence identifies clusters with high vulnerability to multiple pathogens. We identify a candidate group of antigens that are correlated with high overall vulnerability. Our results demonstrate a role for multiplex serology in integrated disease surveillance to guide control strategies for individual and co-endemic pathogens.

Suggested Citation

  • Andrea C. Carcelen & Celso Monjane & Sophie Bérubé & Saki Takahashi & Thebora Sultane & Imelda Chelene & Gretchen Cooley & E. Brook Goodhew & Catriona Patterson & Kevin Tetteh & Manuel Mutambe & Melis, 2025. "Multiplex bead assays enable integrated serological surveillance and reveal cross-pathogen vulnerabilities in Zambezia Province, Mozambique," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62305-9
    DOI: 10.1038/s41467-025-62305-9
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