IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-62229-4.html
   My bibliography  Save this article

Single urinary extracellular vesicle proteomics identifies complement receptor CD35 as a biomarker for sepsis-associated acute kidney injury

Author

Listed:
  • Ning Li

    (Zhong Da Hospital)

  • Tao-Tao Tang

    (Zhong Da Hospital)

  • Menglei Gu

    (Jiangsu Province Hospital of Chinese Medicine)

  • Yu-Qi Fu

    (Zhong Da Hospital)

  • Wei-Wei Qian

    (The Second Affiliated Hospital of Anhui Medical University)

  • Nan Nan Ma

    (The Second Affiliated Hospital of Anhui Medical University)

  • An-Ran Shen

    (Zhong Da Hospital)

  • Tingting Zhu

    (Zhong Da Hospital)

  • Run-Bo Huo

    (Zhongda Hospital)

  • Tao Zhang

    (The Second Affiliated Hospital of Anhui Medical University)

  • Jian-Feng Xie

    (Zhongda Hospital)

  • Lu Zhang

    (Jiangsu Province Hospital of Chinese Medicine)

  • Bi-Cheng Liu

    (Zhong Da Hospital)

  • Lin-Li Lv

    (Zhong Da Hospital)

Abstract

Sepsis-associated acute kidney injury (SA-AKI) portends severe health burden due to significant morbidity and mortality, while early diagnosis remains challenging. In this study, proximity-dependent barcoding assay (PBA) is established to profile the surface proteome of single urinary extracellular vesicle (uEV). Principle uEV clusters with unique function and origination are profiled in SA-AKI in a screening cohort. Complement receptor CD35 on single uEV (CD35-uEV) displays high diagnostic accuracy for SA-AKI (AUC-ROC 0.89 in validation cohort, n = 134). Besides, CD35-uEV enables identification of subclinical AKI (AUC-ROC 0.84 in prospective cohort, n = 72). Moreover, CD35-uEV correlates closely with AKI severity which also predicts persistent AKI (AUC-ROC 0.77), mortality risks (AUC-ROC 0.70) and progression to AKD (AUC-ROC 0.66). Multi-omics profiling reveals that CD35-uEV are predominantly released from injured podocytes exhibiting diminished CD35 expression. Overall, this study identifies a single uEV biomarker related to injured podocyte for early diagnosis and risk stratification of SA-AKI.

Suggested Citation

  • Ning Li & Tao-Tao Tang & Menglei Gu & Yu-Qi Fu & Wei-Wei Qian & Nan Nan Ma & An-Ran Shen & Tingting Zhu & Run-Bo Huo & Tao Zhang & Jian-Feng Xie & Lu Zhang & Bi-Cheng Liu & Lin-Li Lv, 2025. "Single urinary extracellular vesicle proteomics identifies complement receptor CD35 as a biomarker for sepsis-associated acute kidney injury," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62229-4
    DOI: 10.1038/s41467-025-62229-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-62229-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-62229-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62229-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.