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The A-C linker controls centriole structural integrity and duplication

Author

Listed:
  • Lorène Bournonville

    (Department of Molecular and Cellular Biology)

  • Marine. H. Laporte

    (Department of Molecular and Cellular Biology
    Institut NeuroMyoGène)

  • Susanne Borgers

    (Department of Molecular and Cellular Biology)

  • Paul Guichard

    (Department of Molecular and Cellular Biology)

  • Virginie Hamel

    (Department of Molecular and Cellular Biology)

Abstract

Centrioles are evolutionarily conserved barrel-shaped organelles playing crucial roles in cell division and ciliogenesis. These functions are underpinned by specific structural sub-elements whose functions have been under investigation since many years. The A-C linker structure, connecting adjacent microtubule triplets in the proximal region, has remained unexplored due to its unknown composition. Here, using ultrastructure expansion microscopy, we characterized two recently identified A-C linker proteins, CCDC77 and WDR67, and discovered MIIP as an additional A-C linker protein. Our findings reveal that these proteins localize between microtubule triplets at the A-C linker, forming a complex. Depletion of A-C linker components disrupt microtubule triplet cohesion, leading to breakage at the proximal end. Co-removal of the A-C linker and the inner scaffold demonstrates their joint role in maintaining centriole architecture. Moreover, we uncover an unexpected function of the A-C linker in centriole duplication through torus regulation, underscoring the interplay between these protein modules.

Suggested Citation

  • Lorène Bournonville & Marine. H. Laporte & Susanne Borgers & Paul Guichard & Virginie Hamel, 2025. "The A-C linker controls centriole structural integrity and duplication," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-62154-6
    DOI: 10.1038/s41467-025-62154-6
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    References listed on IDEAS

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    1. Vincent Louvel & Romuald Haase & Olivier Mercey & Marine H. Laporte & Thibaut Eloy & Étienne Baudrier & Denis Fortun & Dominique Soldati-Favre & Virginie Hamel & Paul Guichard, 2023. "iU-ExM: nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Laurence Pelletier & Eileen O’Toole & Anne Schwager & Anthony A. Hyman & Thomas Müller-Reichert, 2006. "Centriole assembly in Caenorhabditis elegans," Nature, Nature, vol. 444(7119), pages 619-623, November.
    3. Midori Ohta & Tomoko Ashikawa & Yuka Nozaki & Hiroko Kozuka-Hata & Hidemasa Goto & Masaki Inagaki & Masaaki Oyama & Daiju Kitagawa, 2014. "Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    4. T. Tony Yang & Weng Man Chong & Won-Jing Wang & Gregory Mazo & Barbara Tanos & Zhengmin Chen & Thi Minh Nguyet Tran & Yi-De Chen & Rueyhung Roc Weng & Chia-En Huang & Wann-Neng Jane & Meng-Fu Bryan Ts, 2018. "Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    5. Tae-Sung Kim & Liang Zhang & Jong Ahn & Lingjun Meng & Yang Chen & Eunhye Lee & Jeong Kyu Bang & Jung Mi Lim & Rodolfo Ghirlando & Lixin Fan & Yun-Xing Wang & Bo Yeon Kim & Jung-Eun Park & Kyung S. Le, 2019. "Molecular architecture of a cylindrical self-assembly at human centrosomes," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    6. Pallavi Panda & Mark S. Ladinsky & David M. Glover, 2024. "9-fold symmetry is not essential for centriole elongation and formation of new centriole-like structures," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
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