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Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane

Author

Listed:
  • Tong Chen

    (Huazhong Agricultural University)

  • Aotian Ouyang

    (Huazhong Agricultural University)

  • Jiahui Zou

    (Huazhong Agricultural University)

  • Yi Feng

    (Huazhong Agricultural University)

  • Changsong Wu

    (Huazhong Agricultural University)

  • Meijun Jiang

    (Huazhong Agricultural University)

  • Shaoyu Tu

    (Huazhong Agricultural University)

  • Ling Ding

    (Huazhong Agricultural University)

  • Yanqing Cheng

    (Huazhong Agricultural University)

  • Wenshuo Hu

    (Huazhong Agricultural University)

  • Wei Sheng

    (Huazhong Agricultural University)

  • Yanglin Li

    (Huazhong Agricultural University)

  • Meilin Jin

    (Huazhong Agricultural University
    Frontiers Science Center for Animal Breeding and Sustainable Production)

  • Huanchun Chen

    (Huazhong Agricultural University
    Frontiers Science Center for Animal Breeding and Sustainable Production
    Hubei Hongshan Laboratory
    the Cooperative Innovation Center for Sustainable Pig Production)

  • Hongbo Zhou

    (Huazhong Agricultural University
    Frontiers Science Center for Animal Breeding and Sustainable Production
    Hubei Hongshan Laboratory
    the Cooperative Innovation Center for Sustainable Pig Production)

Abstract

The molecular mechanisms underlying the transport of influenza A virus (IAV) membrane proteins to the cell surface remain largely unclear. In this study, siRNA screening identifies Rab27a as a critical host factor regulating this transport process. GTP-bound Rab27a operates via its effectors, synaptotagmin-like protein 1 (SYTL1) and SYTL4, to facilitate the transport of vesicles carrying viral membrane proteins to the plasma membrane. Absence of Rab27a or SYTL4 does not block the early stages of the IAV life cycle but restricts viral assembly and budding. Notably, silencing SYTL4 provides superior protection in the female mouse IAV infection model. This investigation elucidates the molecular mechanism by which Rab27a and its effectors modulate the transport of IAV membrane proteins, thereby bridging a critical gap in IAV life cycle research and presenting a potential target for the development of antiviral drugs.

Suggested Citation

  • Tong Chen & Aotian Ouyang & Jiahui Zou & Yi Feng & Changsong Wu & Meijun Jiang & Shaoyu Tu & Ling Ding & Yanqing Cheng & Wenshuo Hu & Wei Sheng & Yanglin Li & Meilin Jin & Huanchun Chen & Hongbo Zhou, 2025. "Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61587-3
    DOI: 10.1038/s41467-025-61587-3
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