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BAHCC1 binds H4K20me1 to facilitate the MCM complex loading and DNA replication

Author

Listed:
  • Dongxu Li

    (University of North Carolina at Chapel Hill
    Polyseq Biotech Inc.)

  • Zhi-Min Zhang

    (Riverside
    Jinan University)

  • Liu Mei

    (University of North Carolina at Chapel Hill)

  • Yao Yu

    (Duke University School of Medicine)

  • Yiran Guo

    (Duke University School of Medicine)

  • Samuel G. Mackintosh

    (University of Arkansas for Medical Sciences)

  • Jianbin Chen

    (Riverside)

  • David F. Allison

    (University of North Carolina at Chapel Hill
    Triangle Biotechnology Inc.)

  • Arum Kim

    (Duke University School of Medicine)

  • Aaron J. Storey

    (University of Arkansas for Medical Sciences)

  • Ricky D. Edmondson

    (University of Arkansas for Medical Sciences)

  • Stephanie D. Byrum

    (University of Arkansas for Medical Sciences)

  • Alan J. Tackett

    (University of Arkansas for Medical Sciences)

  • Ling Cai

    (Duke University School of Medicine
    Duke University School of Medicine)

  • Jeanette G. Cook

    (University of North Carolina at Chapel Hill)

  • Jikui Song

    (Riverside)

  • Gang Greg Wang

    (University of North Carolina at Chapel Hill
    Duke University School of Medicine
    Duke University School of Medicine)

Abstract

Mono-methylation of histone H4 lysine 20 (H4K20me1) regulates DNA replication, cell cycle progression and DNA damage repair. How exactly H4K20me1 regulates these biological processes remains unclear. Here, we report that an evolutionarily conserved tandem Tudor domain (TTD) in BAHCC1 (BAHCC1TTD) selectively reads H4K20me1 for facilitating replication origin activation and DNA replication. Our biochemical, structural, genomic and cellular analyses demonstrate that BAHCC1TTD preferentially recognizes H4K20me1 to promote the recruitment of BAHCC1 and its interacting partners, notably Mini-chromosome Maintenance (MCM) complex, to replication origin sites. Combined actions of the H4K20me1-reading BAHCC1 and the H4K20me2-reading Origin Recognition Complex (ORC) ensure genomic loading of MCM for replication. Depletion of BAHCC1, or disruption of the BAHCC1TTD:H4K20me1 interaction, reduces H4K20me1 levels and MCM loading, leading to defects in replication origin activation and cell cycle progression. In summary, this study identifies BAHCC1TTD as an effector transducing H4K20me1 signals into MCM recruitment to promote DNA replication.

Suggested Citation

  • Dongxu Li & Zhi-Min Zhang & Liu Mei & Yao Yu & Yiran Guo & Samuel G. Mackintosh & Jianbin Chen & David F. Allison & Arum Kim & Aaron J. Storey & Ricky D. Edmondson & Stephanie D. Byrum & Alan J. Tacke, 2025. "BAHCC1 binds H4K20me1 to facilitate the MCM complex loading and DNA replication," Nature Communications, Nature, vol. 16(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61284-1
    DOI: 10.1038/s41467-025-61284-1
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    References listed on IDEAS

    as
    1. Alex J. Kuo & Jikui Song & Peggie Cheung & Satoko Ishibe-Murakami & Sayumi Yamazoe & James K. Chen & Dinshaw J. Patel & Or Gozani, 2012. "The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier–Gorlin syndrome," Nature, Nature, vol. 484(7392), pages 115-119, April.
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