IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-61043-2.html
   My bibliography  Save this article

Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma

Author

Listed:
  • Lakkhana Sadaow

    (Khon Kaen University)

  • Rutchanee Rodpai

    (Khon Kaen University)

  • Michael J. Smout

    (James Cook University)

  • Rie Nakajima

    (University of California Irvine)

  • Patcharaporn Boonroumkaew

    (Khon Kaen University)

  • Javier Sotillo

    (Instituto de Salud Carlos III)

  • Bemnet A. Tedla

    (James Cook University)

  • Vor Luvira

    (Khon Kaen University)

  • Amnat Kitkhuandee

    (Khon Kaen University)

  • Krisada Paonariang

    (Khon Kaen University)

  • Wattana Sukeepaisarnjaroen

    (Khon Kaen University)

  • Hiroshi Yamasaki

    (National Institute of Infectious Diseases)

  • Sutas Suttiprapa

    (Khon Kaen University)

  • Thewarach Laha

    (Khon Kaen University)

  • Banchob Sripa

    (Khon Kaen University)

  • Rafael Assis

    (University of California Irvine)

  • Aarti Jain

    (University of California Irvine)

  • Wannaporn Ittiprasert

    (George Washington University)

  • Victoria H. Mann

    (George Washington University)

  • Yide Wong

    (James Cook University)

  • Philip L. Felgner

    (University of California Irvine)

  • Wanchai Maleewong

    (Khon Kaen University)

  • Paul J. Brindley

    (George Washington University)

  • Alex Loukas

    (James Cook University)

  • Pewpan M. Intapan

    (Khon Kaen University)

Abstract

Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.

Suggested Citation

  • Lakkhana Sadaow & Rutchanee Rodpai & Michael J. Smout & Rie Nakajima & Patcharaporn Boonroumkaew & Javier Sotillo & Bemnet A. Tedla & Vor Luvira & Amnat Kitkhuandee & Krisada Paonariang & Wattana Suke, 2025. "Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61043-2
    DOI: 10.1038/s41467-025-61043-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-61043-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-61043-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61043-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.