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Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma

Author

Listed:
  • Lakkhana Sadaow

    (Khon Kaen University)

  • Rutchanee Rodpai

    (Khon Kaen University)

  • Michael J. Smout

    (James Cook University)

  • Rie Nakajima

    (University of California Irvine)

  • Patcharaporn Boonroumkaew

    (Khon Kaen University)

  • Javier Sotillo

    (Instituto de Salud Carlos III)

  • Bemnet A. Tedla

    (James Cook University)

  • Vor Luvira

    (Khon Kaen University)

  • Amnat Kitkhuandee

    (Khon Kaen University)

  • Krisada Paonariang

    (Khon Kaen University)

  • Wattana Sukeepaisarnjaroen

    (Khon Kaen University)

  • Hiroshi Yamasaki

    (National Institute of Infectious Diseases)

  • Sutas Suttiprapa

    (Khon Kaen University)

  • Thewarach Laha

    (Khon Kaen University)

  • Banchob Sripa

    (Khon Kaen University)

  • Rafael Assis

    (University of California Irvine)

  • Aarti Jain

    (University of California Irvine)

  • Wannaporn Ittiprasert

    (George Washington University)

  • Victoria H. Mann

    (George Washington University)

  • Yide Wong

    (James Cook University)

  • Philip L. Felgner

    (University of California Irvine)

  • Wanchai Maleewong

    (Khon Kaen University)

  • Paul J. Brindley

    (George Washington University)

  • Alex Loukas

    (James Cook University)

  • Pewpan M. Intapan

    (Khon Kaen University)

Abstract

Sensitive diagnostics are needed to improve management and surveillance of opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma (CCA) throughout East Asia. Herein we generate and screen an Opisthorchis viverrini recombinant secreted proteome to identity antibody biomarkers of liver fluke infection and CCA with sera from study participants in endemic populations and evaluate their utility as point-of-care immunochromatographic tests (PoC-ICTs). We incorporate two of the most promising antigens from the proteome array screen, P1 and P9, into PoC-ICTs to further validate their diagnostic performance. The P9-IgG4 PoC-ICT is superior amongst the single recombinant antigen tests for diagnosing fluke infection as well as fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs. Here we identify two biomarkers of O. viverrini infection and infection-associated CCA that could form the basis of novel antibody serodiagnostic tests for human liver fluke infection and associated cancer.

Suggested Citation

  • Lakkhana Sadaow & Rutchanee Rodpai & Michael J. Smout & Rie Nakajima & Patcharaporn Boonroumkaew & Javier Sotillo & Bemnet A. Tedla & Vor Luvira & Amnat Kitkhuandee & Krisada Paonariang & Wattana Suke, 2025. "Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-61043-2
    DOI: 10.1038/s41467-025-61043-2
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    References listed on IDEAS

    as
    1. Sangtae Kim & Pavel A. Pevzner, 2014. "MS-GF+ makes progress towards a universal database search tool for proteomics," Nature Communications, Nature, vol. 5(1), pages 1-10, December.
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