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Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles

Author

Listed:
  • Yisha Wang

    (Zhejiang University School of Medicine)

  • Gan Luo

    (Zhejiang University School of Medicine)

  • Haiyang Wang

    (Zhejiang University School of Medicine)

  • Yue Zheng

    (Zhejiang University School of Medicine)

  • Xiao Xu

    (Hangzhou Medical College)

  • Wenbin Zhou

    (Zhejiang University School of Medicine)

  • Junrong Lin

    (Zhejiang University School of Medicine)

  • Baocheng Chen

    (Zhejiang University School of Medicine)

  • Yangfu Guo

    (Zhejiang University School of Medicine
    Henan University)

  • Yifeng Jin

    (Zhejiang University School of Medicine)

  • Meihua Sui

    (Zhejiang University School of Medicine
    Zhejiang University School of Medicine)
    Zhejiang University)

Abstract

Little is known about how cell cycle and autophagy, two fundamental life processes, affect cellular accumulation of nanoparticles. What’s even more tough is that several long-lasting methodological barriers have hindered the progress of related research. Here we firstly show the construction of a multi-functional platform for overcoming existing methodological obstacles through integrating multiple technical approaches including autophagy-related gene 7 knockout to specifically block autophagy, PIP-FUCCI transfection and mitotic shake-off to thoroughly separate cell cycle phases, and 3D reconstruction to stereoscopically evaluate cellular accumulation of nanoparticles. Further application of this platform reveals that after a 2-hour incubation of lipid-based nanoparticles, G2-phase and M-phase cells, two populations previously muddled up together as G2/M-phase cells, respectively exhibited the maximum and minimum nanoparticle accumulation. Meanwhile, our data preliminarily suggest enhanced nanoparticle accumulation by autophagy blockade. Besides cell cycle and autophagy, comprehensive statistical analyses reveal a close association between cellular accumulation of nanoparticles and nanoparticle type. This study not only provides a valuable technical strategy, but uncovers important characteristics of cellular accumulation of nanoparticles, offering new insights for optimization and application of nanomedicines.

Suggested Citation

  • Yisha Wang & Gan Luo & Haiyang Wang & Yue Zheng & Xiao Xu & Wenbin Zhou & Junrong Lin & Baocheng Chen & Yangfu Guo & Yifeng Jin & Meihua Sui, 2025. "Evaluating cell cycle- and autophagy-associated cellular accumulation of lipid-based nanoparticles," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60962-4
    DOI: 10.1038/s41467-025-60962-4
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    References listed on IDEAS

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