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Production of live monkeys and their genetically matched embryonic stem cells from single embryos

Author

Listed:
  • Chenyang Si

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Ran Zhu

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research
    Southwest United Graduate School)

  • Junmo Wu

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Zhenzhen Chen

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Zengli Tang

    (Kunming University of Science and Technology
    Southwest United Graduate School)

  • Zuoyao Li

    (Kunming University of Science and Technology)

  • Yu Kang

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Lifeng Xiang

    (The First People’s Hospital of Yunnan Province; The Affiliated Hospital of Kunming University of Science and Technology)

  • Jiawei Zuo

    (Kunming University of Science and Technology
    Southwest United Graduate School)

  • Pengpeng Yang

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Chu Chu

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Shanshan Yang

    (Kunming University of Science and Technology)

  • Zifan Li

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Lu Zhao

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Xinglong Chen

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Youwei Pu

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Baohong Tian

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Zhaohui Yang

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Weizhi Ji

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Shaoxing Dai

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

  • Yuyu Niu

    (Kunming University of Science and Technology
    Yunnan Key Laboratory of Primate Biomedical Research)

Abstract

Immune rejection poses a challenge in stem cell therapy, especially with allogeneic embryonic stem cells (ESCs). Non-human primates offer a promising avenue for developing genetically matched ESCs for regenerative medicine. Here, we successfully derive three live monkeys and their genetically matched autologous ESCs (aESCs) using embryo splitting. Additionally, from fibroblasts of one of these monkeys, we generate induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cells (ntESCs), creating a set of genetically matched aESCs, iPSCs, and ntESCs. Single-cell RNA-seq analysis reveals that aESCs potentially exhibit reduced heterogeneity, lower transcriptional noise, and enhanced genomic stability compared to iPSCs and ntESCs. Furthermore, we successfully derive ESCs from human split embryos, highlighting the potential for obtaining human aESCs. Collectively, our study offers an avenue for establishing autologous pluripotent stem cells and provides the theoretical basis as well as research model for further application of aESCs in human regenerative medicine.

Suggested Citation

  • Chenyang Si & Ran Zhu & Junmo Wu & Zhenzhen Chen & Zengli Tang & Zuoyao Li & Yu Kang & Lifeng Xiang & Jiawei Zuo & Pengpeng Yang & Chu Chu & Shanshan Yang & Zifan Li & Lu Zhao & Xinglong Chen & Youwei, 2025. "Production of live monkeys and their genetically matched embryonic stem cells from single embryos," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60694-5
    DOI: 10.1038/s41467-025-60694-5
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