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Photochemical synthesis of natural lipids in artificial and living cells

Author

Listed:
  • Peng Ji

    (University of California, San Diego)

  • Alexander Harjung

    (University of California, San Diego)

  • Caroline H. Knittel

    (University of California, San Diego)

  • Alessandro Fracassi

    (University of California, San Diego)

  • Jiyue Chen

    (University of California, San Diego)

  • Roberto J. Brea

    (University of California, San Diego)

  • Neal K. Devaraj

    (University of California, San Diego)

Abstract

Lipid synthesis plays a central role in cell structure, signaling, and metabolism. A general method for the abiogenesis of natural lipids could transform the development of lifelike artificial cells and unlock new ways to explore lipid functions in living cells. Here, we demonstrate the abiotic formation of natural lipids in water using visible-light-driven photoredox chemistry. Radical-mediated coupling of hydrocarbon tails to polar single-chain precursors yields lipids identical to those enzymatically formed. Spatiotemporally controlled lipid generation promotes de novo vesicle formation, growth, and division. Lipid synthesis can be driven by RNA aptamers that specifically bind and activate photocatalysts, establishing a direct link between abiotic lipid metabolism and nucleic acid sequence. Light-mediated assembly of bioactive lipids can take place in living cells, triggering signaling events such as apoptosis and protein kinase C (PKC) activation. Our finding that photochemical lipid synthesis can be driven by simple genetic elements could be the starting point for developing protocells capable of Darwinian evolution. Additionally, the ability to generate specific membrane lipids in living cells with precise spatiotemporal control will advance studies on how lipid structure influences cellular function.

Suggested Citation

  • Peng Ji & Alexander Harjung & Caroline H. Knittel & Alessandro Fracassi & Jiyue Chen & Roberto J. Brea & Neal K. Devaraj, 2025. "Photochemical synthesis of natural lipids in artificial and living cells," Nature Communications, Nature, vol. 16(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60358-4
    DOI: 10.1038/s41467-025-60358-4
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    References listed on IDEAS

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    1. Duco Blanken & David Foschepoth & Adriana Calaça Serrão & Christophe Danelon, 2020. "Genetically controlled membrane synthesis in liposomes," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    2. Kensuke Kurihara & Yusaku Okura & Muneyuki Matsuo & Taro Toyota & Kentaro Suzuki & Tadashi Sugawara, 2015. "A recursive vesicle-based model protocell with a primitive model cell cycle," Nature Communications, Nature, vol. 6(1), pages 1-7, December.
    3. Shashank Dadsena & Svenja Bockelmann & John G. M. Mina & Dina G. Hassan & Sergei Korneev & Guilherme Razzera & Helene Jahn & Patrick Niekamp & Dagmar Müller & Markus Schneider & Fikadu G. Tafesse & Si, 2019. "Ceramides bind VDAC2 to trigger mitochondrial apoptosis," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    4. Brian Josephson & Charlie Fehl & Patrick G. Isenegger & Simon Nadal & Tom H. Wright & Adeline W. J. Poh & Ben J. Bower & Andrew M. Giltrap & Lifu Chen & Christopher Batchelor-McAuley & Grace Roper & O, 2020. "Light-driven post-translational installation of reactive protein side chains," Nature, Nature, vol. 585(7826), pages 530-537, September.
    5. Joost C.M. Holthuis & Anant K. Menon, 2014. "Lipid landscapes and pipelines in membrane homeostasis," Nature, Nature, vol. 510(7503), pages 48-57, June.
    6. Alexis Autour & Sunny Jeng & Adam Cawte & Amir Abdolahzadeh & Angela Galli & Shanker S. S. Panchapakesan & David Rueda & Michael Ryckelynck & Peter J. Unrau, 2018. "Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
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