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Ciprofloxacin resistance rapidly declines in nfxB defective clinical strains of Pseudomonas aeruginosa

Author

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  • Sara Hernando-Amado

    (Centro Nacional de Biotecnología. CSIC)

  • Pablo Laborda

    (Rigshospitalet)

  • Ruggero Rosa

    (Rigshospitalet
    Technical University of Denmark)

  • Søren Molin

    (Technical University of Denmark)

  • Helle Krogh Johansen

    (Rigshospitalet
    University of Copenhagen)

  • José Luis Martínez

    (Centro Nacional de Biotecnología. CSIC)

Abstract

Antibiotic-resistant bacteria could be tackled by identifying trade-offs of evolution, such as high fitness costs, which may be harnessed to force reversion to susceptibility. A decline in antimicrobial resistance can occur through compensatory mutations or by genetic reversion to the wild-type allele, which reduce fitness costs associated with resistance. We analyse here the impact of antibiotic-free environments on declining ciprofloxacin resistance in eight nfxB defective clinical strains of Pseudomonas aeruginosa spanning varied clone types and ciprofloxacin resistance levels. Ciprofloxacin resistance declines in just 100 generations, which is mainly caused by newly acquired mutations in the genes encoding the overproduced efflux pump MexCD-OprJ and not by the reversion of nfxB mutations of the parental strains. The rapid reversion of ciprofloxacin resistance in P. aeruginosa suggests the potential for reusing this essential antibiotic and underlines the need to implement evolution-based approaches against nfxB defective resistant mutant strains.

Suggested Citation

  • Sara Hernando-Amado & Pablo Laborda & Ruggero Rosa & Søren Molin & Helle Krogh Johansen & José Luis Martínez, 2025. "Ciprofloxacin resistance rapidly declines in nfxB defective clinical strains of Pseudomonas aeruginosa," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60330-2
    DOI: 10.1038/s41467-025-60330-2
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