IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-60302-6.html
   My bibliography  Save this article

Bioenergetic stress potentiates antimicrobial resistance and persistence

Author

Listed:
  • Barry Li

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Shivani Srivastava

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Mustafa Shaikh

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Gautam Mereddy

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Madison R. Garcia

    (Rutgers New Jersey Medical School
    The State University of New Jersey)

  • Eric N. Chiles

    (The State University of New Jersey)

  • Avi Shah

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Boatema Ofori-Anyinam

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Ting-Yu Chu

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Nicole J. Cheney

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

  • Douglas McCloskey

    (Technical University of Denmark
    BioMed X Institute)

  • Xiaoyang Su

    (The State University of New Jersey
    Rutgers Robert Wood Johnson Medical School)

  • Jason H. Yang

    (Rutgers New Jersey Medical School
    Rutgers New Jersey Medical School)

Abstract

The bactericidal action of some antibiotics is associated with increased ATP consumption, cellular respiration, and reactive oxygen species (ROS) formation. Here, we investigate the effects of ‘bioenergetic stress’, induced by constitutive hydrolysis of ATP and NADH, on antibiotic efficacy in Escherichia coli. We show that bioenergetic stress potentiates the evolution of antibiotic resistance via enhanced ROS production, mutagenic break repair, and transcription-coupled repair. In addition, bioenergetic stress potentiates antibiotic persistence via the stringent response. We propose a model in which the balance between ATP consumption versus production regulates antibiotic resistance and persistence.

Suggested Citation

  • Barry Li & Shivani Srivastava & Mustafa Shaikh & Gautam Mereddy & Madison R. Garcia & Eric N. Chiles & Avi Shah & Boatema Ofori-Anyinam & Ting-Yu Chu & Nicole J. Cheney & Douglas McCloskey & Xiaoyang , 2025. "Bioenergetic stress potentiates antimicrobial resistance and persistence," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60302-6
    DOI: 10.1038/s41467-025-60302-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-60302-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-60302-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Wout Megchelenbrink & Martijn Huynen & Elena Marchiori, 2014. "optGpSampler : An Improved Tool for Uniformly Sampling the Solution-Space of Genome-Scale Metabolic Networks," PLOS ONE, Public Library of Science, vol. 9(2), pages 1-8, February.
    2. Theresa C. Barrett & Wendy W. K. Mok & Allison M. Murawski & Mark P. Brynildsen, 2019. "Enhanced antibiotic resistance development from fluoroquinolone persisters after a single exposure to antibiotic," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Alexandr Safatov & Irina Andreeva & Galina Buryak & Olesia Ohlopkova & Sergei Olkin & Larisa Puchkova & Irina Reznikova & Nadezda Solovyanova & Boris Belan & Mikhail Panchenko & Denis Simonenkov, 2020. "How Has the Hazard to Humans of Microorganisms Found in Atmospheric Aerosol in the South of Western Siberia Changed over 10 Years?," IJERPH, MDPI, vol. 17(5), pages 1-19, March.
    2. Cyril Bachelard & Apostolos Chalkis & Vissarion Fisikopoulos & Elias Tsigaridas, 2024. "Randomized Control in Performance Analysis and Empirical Asset Pricing," Papers 2403.00009, arXiv.org.
    3. Shirin Fallahi & Hans J Skaug & Guttorm Alendal, 2020. "A comparison of Monte Carlo sampling methods for metabolic network models," PLOS ONE, Public Library of Science, vol. 15(7), pages 1-24, July.
    4. Marcelo Rivas-Astroza & Raúl Conejeros, 2020. "Metabolic flux configuration determination using information entropy," PLOS ONE, Public Library of Science, vol. 15(12), pages 1-19, December.
    5. Asli Sahin & Daniel R. Weilandt & Vassily Hatzimanikatis, 2023. "Optimal enzyme utilization suggests that concentrations and thermodynamics determine binding mechanisms and enzyme saturations," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    6. Erica J. Zheng & Ian W. Andrews & Alexandra T. Grote & Abigail L. Manson & Miguel A. Alcantar & Ashlee M. Earl & James J. Collins, 2022. "Modulating the evolutionary trajectory of tolerance using antibiotics with different metabolic dependencies," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-60302-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.