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C. elegans SSNA-1 is required for the structural integrity of centrioles and bipolar spindle assembly

Author

Listed:
  • Jason A. Pfister

    (National Institutes of Health)

  • Lorenzo Agostini

    (National Institutes of Health)

  • Lorène Bournonville

    (University of Geneva)

  • Aurélien Perrier

    (University of Geneva)

  • Prabhu Sankaralingam

    (National Institutes of Health)

  • Zachary G. Bell

    (National Institutes of Health)

  • Virginie Hamel

    (University of Geneva)

  • Paul Guichard

    (University of Geneva)

  • Christian Biertümpfel

    (National Institutes of Health)

  • Naoko Mizuno

    (National Institutes of Health
    National Institutes of Health)

  • Kevin F. O’Connell

    (National Institutes of Health)

Abstract

Centrioles play key roles in mitotic spindle assembly. Once assembled, centrioles exhibit long-term stability, but how stability is achieved and how it is regulated are not completely understood. In this study we show that SSNA-1, the Caenorhabditis elegans ortholog of Sjogren’s Syndrome Nuclear Antigen 1, is a constituent of centrioles and centriole satellite-like structures. A deletion of ssna-1 results in the formation of extra centrioles. We show that SSNA-1 genetically interacts with the centriole stability factor SAS-1 and is required post assembly for centriole structural integrity. In SSNA-1’s absence, centrioles assemble but fracture leading to extra spindle poles. However, if the efficiency of cartwheel assembly is reduced, the absence of SSNA-1 results in daughter centriole loss and monopolar spindles, indicating that the cartwheel and SSNA-1 cooperate to stabilize centrioles during assembly. Our work thus shows that SSNA-1 contributes to centriole stability during and after assembly, thereby ensuring proper centriole number.

Suggested Citation

  • Jason A. Pfister & Lorenzo Agostini & Lorène Bournonville & Aurélien Perrier & Prabhu Sankaralingam & Zachary G. Bell & Virginie Hamel & Paul Guichard & Christian Biertümpfel & Naoko Mizuno & Kevin F., 2025. "C. elegans SSNA-1 is required for the structural integrity of centrioles and bipolar spindle assembly," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59939-0
    DOI: 10.1038/s41467-025-59939-0
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    References listed on IDEAS

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    1. Anil Shukla & Dong Kong & Meena Sharma & Valentin Magidson & Jadranka Loncarek, 2015. "Plk1 relieves centriole block to reduplication by promoting daughter centriole maturation," Nature Communications, Nature, vol. 6(1), pages 1-13, November.
    2. Laurence Pelletier & Eileen O’Toole & Anne Schwager & Anthony A. Hyman & Thomas Müller-Reichert, 2006. "Centriole assembly in Caenorhabditis elegans," Nature, Nature, vol. 444(7119), pages 619-623, November.
    3. Meng-Fu Bryan Tsou & Tim Stearns, 2006. "Mechanism limiting centrosome duplication to once per cell cycle," Nature, Nature, vol. 442(7105), pages 947-951, August.
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