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Direct evaluation of antiplatelet therapy in coronary artery disease by comprehensive image-based profiling of circulating platelets

Author

Listed:
  • Kazutoshi Hirose

    (The University of Tokyo)

  • Satoshi Kodera

    (The University of Tokyo)

  • Masako Nishikawa

    (The University of Tokyo)

  • Masataka Sato

    (The University of Tokyo)

  • Yuqi Zhou

    (The University of Tokyo)

  • Hongqian Zhang

    (The University of Tokyo)

  • Shun Minatsuki

    (The University of Tokyo)

  • Junichi Ishida

    (The University of Tokyo)

  • Norifumi Takeda

    (The University of Tokyo)

  • Huidong Wang

    (The University of Tokyo)

  • Chuiming Kong

    (The University of Tokyo)

  • Yunjie Deng

    (The University of Tokyo)

  • Junyu Chen

    (The University of Tokyo)

  • Chenqi Zhang

    (The University of Tokyo)

  • Jun Akita

    (The University of Tokyo)

  • Yuma Ibayashi

    (The University of Tokyo)

  • Ruoxi Yang

    (The University of Tokyo)

  • Hiroshi Kanno

    (The University of Tokyo
    Tohoku University Graduate School of Medicine)

  • Nao Nitta

    (CYBO Inc)

  • Takeaki Sugimura

    (CYBO Inc)

  • Norihiko Takeda

    (The University of Tokyo)

  • Makoto Kurano

    (The University of Tokyo)

  • Yutaka Yatomi

    (The University of Tokyo
    International University of Health and Welfare Graduate School)

  • Keisuke Goda

    (The University of Tokyo
    CYBO Inc
    University of California
    Wuhan University)

Abstract

Coronary artery disease (CAD) is a leading cause of death globally. Antiplatelet therapy remains crucial in preventing and treating CAD-associated thrombotic complications, but it concurrently amplifies the risk of bleeding. Unfortunately, traditional platelet function measurement methods cannot directly evaluate its efficacy and safety. Here we demonstrate comprehensive image-based profiling of circulating platelets to directly observe thrombotic conditions and assess antiplatelet therapy in CAD patients. Deep learning-based analysis of whole blood samples from 207 CAD patients revealed elevated concentrations of circulating platelet aggregates, especially in acute versus chronic coronary syndrome patients. It also indicated a regimen-dependent reduction in these concentrations upon treatment with antiplatelet drugs, thereby verifying the direct efficacy of the therapy. Notably, consistent concentrations of these aggregates were found in both venous and arterial blood, suggesting venous blood as a reliable therapy efficacy indicator, despite CAD’s arterial nature. These findings support personalized and improved antiplatelet therapy in CAD management.

Suggested Citation

  • Kazutoshi Hirose & Satoshi Kodera & Masako Nishikawa & Masataka Sato & Yuqi Zhou & Hongqian Zhang & Shun Minatsuki & Junichi Ishida & Norifumi Takeda & Huidong Wang & Chuiming Kong & Yunjie Deng & Jun, 2025. "Direct evaluation of antiplatelet therapy in coronary artery disease by comprehensive image-based profiling of circulating platelets," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59664-8
    DOI: 10.1038/s41467-025-59664-8
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    References listed on IDEAS

    as
    1. Masako Nishikawa & Hiroshi Kanno & Yuqi Zhou & Ting-Hui Xiao & Takuma Suzuki & Yuma Ibayashi & Jeffrey Harmon & Shigekazu Takizawa & Kotaro Hiramatsu & Nao Nitta & Risako Kameyama & Walker Peterson & , 2021. "Massive image-based single-cell profiling reveals high levels of circulating platelet aggregates in patients with COVID-19," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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