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An RNA helicase coordinates with iron signal regulators to alleviate chilling stress in Arabidopsis

Author

Listed:
  • Yingying Xing

    (China Agricultural University)

  • Yawen Li

    (Chinese Academy of Sciences)

  • Xinmeng Gui

    (Shanghai Normal University)

  • Xianyu Zhang

    (China Agricultural University)

  • Qian Hu

    (China Agricultural University)

  • Qiqi Zhao

    (China Agricultural University)

  • Yongli Qiao

    (Shanghai Normal University)

  • Ning Xu

    (China Agricultural University)

  • Jun Liu

    (China Agricultural University)

Abstract

Chilling stress is one of the major environmental stresses that restrains plant development and growth. Our previous study showed that a potential iron sensor BTS (BRUTUS) was involved in temperature response in Arabidopsis plants. However, whether plant iron homeostasis is involved in plant response to temperature fluctuation is not known. In this study, we discover that BTS mutant bts-2 is sensitive to chilling stress, and the sensitivity is attributed to the accumulation of iron. The suppressor screening of bts-2 led to the discovery of RH24, a DEAD-box RNA helicase, that fully suppresses bts-2 chilling sensitivity. RH24 is accumulated under low temperatures, where it unwinds the iron regulator ILR3 (IAA-leucine resistant 3) mRNA and increases the ILR3 protein levels. Intriguingly, RH24 sequesters ILR3 in phase-separated condensates to reduce ILR3-mediated iron overload, and BTS or cold treatments further facilitated the condensate formation. Therefore, RH24 and BTS coordinately control ILR3 to reduce iron uptake under chilling stress. Our findings reveal that the RNA helicase RH24 and BTS finetunes ILR3 to maintain plant iron homeostasis in response to temperature fluctuations.

Suggested Citation

  • Yingying Xing & Yawen Li & Xinmeng Gui & Xianyu Zhang & Qian Hu & Qiqi Zhao & Yongli Qiao & Ning Xu & Jun Liu, 2025. "An RNA helicase coordinates with iron signal regulators to alleviate chilling stress in Arabidopsis," Nature Communications, Nature, vol. 16(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-59334-9
    DOI: 10.1038/s41467-025-59334-9
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    References listed on IDEAS

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    1. Ling Xu & Zhaobin Dong & Dawn Chiniquy & Grady Pierroz & Siwen Deng & Cheng Gao & Spencer Diamond & Tuesday Simmons & Heidi M.-L. Wipf & Daniel Caddell & Nelle Varoquaux & Mary A. Madera & Robert Hutm, 2021. "Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    2. Zhenwei Zhang & Norbert Rigo & Olexandr Dybkov & Jean-Baptiste Fourmann & Cindy L. Will & Vinay Kumar & Henning Urlaub & Holger Stark & Reinhard Lührmann, 2021. "Structural insights into how Prp5 proofreads the pre-mRNA branch site," Nature, Nature, vol. 596(7871), pages 296-300, August.
    3. Igor Martianov & Aroul Ramadass & Ana Serra Barros & Natalie Chow & Alexandre Akoulitchev, 2007. "Repression of the human dihydrofolate reductase gene by a non-coding interfering transcript," Nature, Nature, vol. 445(7128), pages 666-670, February.
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