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A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors

Author

Listed:
  • Estelle Leroy

    (Institute for Integrative Biology of the Cell (I2BC))

  • Drice Challal

    (Institute for Integrative Biology of the Cell (I2BC)
    Institut de Biologie Physico-Chimique)

  • Stéphane Pelletier

    (Institute for Integrative Biology of the Cell (I2BC))

  • Coralie Goncalves

    (Institut Jacques Monod)

  • Alexandra Menant

    (Institute for Integrative Biology of the Cell (I2BC))

  • Virginie Marchand

    (Epitranscriptomics and RNA sequencing (EpiRNA-Seq) Core Facility (SMP IBSLor) and UMR7365 IMoPA CNRS)

  • Yan Jaszczyszyn

    (Institute for Integrative Biology of the Cell (I2BC))

  • Erwin Dijk

    (Institute for Integrative Biology of the Cell (I2BC))

  • Delphine Naquin

    (Institute for Integrative Biology of the Cell (I2BC))

  • Jessica Andreani

    (Institute for Integrative Biology of the Cell (I2BC))

  • Yuri Motorin

    (Epitranscriptomics and RNA sequencing (EpiRNA-Seq) Core Facility (SMP IBSLor) and UMR7365 IMoPA CNRS)

  • Benoit Palancade

    (Institut Jacques Monod)

  • Mathieu Rougemaille

    (Institute for Integrative Biology of the Cell (I2BC))

Abstract

Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic and post-transcriptional levels. However, the extent of their regulatory potential and the underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in the fission yeast long non-coding RNA mamRNA and carrying two independent cellular functions. On the one hand, snR107 guides site-specific 25S rRNA 2’-O-methylation and promotes pre-rRNA processing and 60S subunit biogenesis. On the other hand, snR107 associates with the gametogenic RNA-binding proteins Mmi1 and Mei2, mediating their reciprocal inhibition and restricting meiotic gene expression during sexual differentiation. Both functions require distinct cis-motifs within snR107, including a conserved 2’-O-methylation guiding sequence. Together, our results position snR107 as a dual regulator of rRNA modification and gametogenesis effectors, expanding our vision on the non-canonical functions exerted by snoRNAs in cell fate decisions.

Suggested Citation

  • Estelle Leroy & Drice Challal & Stéphane Pelletier & Coralie Goncalves & Alexandra Menant & Virginie Marchand & Yan Jaszczyszyn & Erwin Dijk & Delphine Naquin & Jessica Andreani & Yuri Motorin & Benoi, 2025. "A bifunctional snoRNA with separable activities in guiding rRNA 2’-O-methylation and scaffolding gametogenesis effectors," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58664-y
    DOI: 10.1038/s41467-025-58664-y
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