IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-58509-8.html
   My bibliography  Save this article

Nanopore sensing of protein and peptide conformation for point-of-care applications

Author

Listed:
  • Laura Ratinho

    (Université Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE)

  • Nathan Meyer

    (Université Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE)

  • Sandra Greive

    (DreamPore S.A.S.)

  • Benjamin Cressiot

    (Université Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE)

  • Juan Pelta

    (Université Paris-Saclay, Univ Evry, CY Cergy Paris Université, CNRS, LAMBE)

Abstract

The global population’s aging and growth will likely result in an increase in chronic aging-related diseases. Early diagnosis could improve the medical care and quality of life. Many diseases are linked to misfolding or conformational changes in biomarker peptides and proteins, which affect their function and binding properties. Current clinical methods struggle to detect and quantify these changes. Therefore, there is a need for sensitive conformational sensors that can detect low-concentration analytes in biofluids. Nanopore electrical detection has shown potential in sensing subtle protein and peptide conformation changes. This technique can detect single molecules label-free while distinguishing shape or physicochemical property changes. Its proven sensitivity makes nanopore sensing technology promising for ultra-sensitive, personalized point-of-care devices. We focus on the capability of nanopore sensing for detecting and quantifying conformational modifications and enantiomers in biomarker proteins and peptides and discuss this technology as a solution to future societal health challenges.

Suggested Citation

  • Laura Ratinho & Nathan Meyer & Sandra Greive & Benjamin Cressiot & Juan Pelta, 2025. "Nanopore sensing of protein and peptide conformation for point-of-care applications," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58509-8
    DOI: 10.1038/s41467-025-58509-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-58509-8
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-025-58509-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Christopher M. Dobson, 2003. "Protein folding and misfolding," Nature, Nature, vol. 426(6968), pages 884-890, December.
    2. Keisuke Motone & Daphne Kontogiorgos-Heintz & Jasmine Wee & Kyoko Kurihara & Sangbeom Yang & Gwendolin Roote & Oren E. Fox & Yishu Fang & Melissa Queen & Mattias Tolhurst & Nicolas Cardozo & Miten Jai, 2024. "Multi-pass, single-molecule nanopore reading of long protein strands," Nature, Nature, vol. 633(8030), pages 662-669, September.
    3. Guangdun Peng & Shengbao Suo & Guizhong Cui & Fang Yu & Ran Wang & Jun Chen & Shirui Chen & Zhiwen Liu & Guoyu Chen & Yun Qian & Patrick P. L. Tam & Jing-Dong J. Han & Naihe Jing, 2019. "Molecular architecture of lineage allocation and tissue organization in early mouse embryo," Nature, Nature, vol. 572(7770), pages 528-532, August.
    4. Gang Huang & Arnout Voet & Giovanni Maglia, 2019. "FraC nanopores with adjustable diameter identify the mass of opposite-charge peptides with 44 dalton resolution," Nature Communications, Nature, vol. 10(1), pages 1-10, December.
    5. Nicole Stéphanie Galenkamp & Misha Soskine & Jos Hermans & Carsten Wloka & Giovanni Maglia, 2018. "Direct electrical quantification of glucose and asparagine from bodily fluids using nanopores," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    6. Mohammad Ahmad & Jeung-Hoi Ha & Lauren A. Mayse & Maria F. Presti & Aaron J. Wolfe & Kelsey J. Moody & Stewart N. Loh & Liviu Movileanu, 2023. "A generalizable nanopore sensor for highly specific protein detection at single-molecule precision," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    7. Ilaria Bellantuono, 2018. "Find drugs that delay many diseases of old age," Nature, Nature, vol. 554(7692), pages 293-295, February.
    8. Fabien Piguet & Hadjer Ouldali & Manuela Pastoriza-Gallego & Philippe Manivet & Juan Pelta & Abdelghani Oukhaled, 2018. "Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore," Nature Communications, Nature, vol. 9(1), pages 1-13, December.
    9. Gang Huang & Kherim Willems & Misha Soskine & Carsten Wloka & Giovanni Maglia, 2017. "Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores," Nature Communications, Nature, vol. 8(1), pages 1-11, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Fushi Wang & Chunxiao Zhao & Pinlong Zhao & Fanfan Chen & Dan Qiao & Jiandong Feng, 2023. "MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    2. Smrithi Krishnan R & Kalyanashis Jana & Amina H. Shaji & Karthika S. Nair & Anjali Devi Das & Devika Vikraman & Harsha Bajaj & Ulrich Kleinekathöfer & Kozhinjampara R. Mahendran, 2022. "Assembly of transmembrane pores from mirror-image peptides," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. repec:plo:pcbi00:1000492 is not listed on IDEAS
    4. Nicholas J. Schork & Laura H. Goetz, 2025. "From precision interventions to precision health," Nature Communications, Nature, vol. 16(1), pages 1-11, December.
    5. Arthur Fischbach & Angela Johns & Kara L. Schneider & Xinxin Hao & Peter Tessarz & Thomas Nyström, 2023. "Artificial Hsp104-mediated systems for re-localizing protein aggregates," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    6. Haipeng Fu & Tingyu Wang & Xiaohui Kong & Kun Yan & Yang Yang & Jingyi Cao & Yafei Yuan & Nan Wang & Kehkooi Kee & Zhi John Lu & Qiaoran Xi, 2022. "A Nodal enhanced micropeptide NEMEP regulates glucose uptake during mesendoderm differentiation of embryonic stem cells," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    7. Jincan Ke & Jian Xu & Jia Liu & Yumeng Yang & Chenkai Guo & Bingbing Xie & Guizhong Cui & Guangdun Peng & Shengbao Suo, 2025. "High-resolution mapping of single cells in spatial context," Nature Communications, Nature, vol. 16(1), pages 1-21, December.
    8. Yun-Dong Yin & Yu-Wei Zhang & Xi-Tong Song & Jun Hu & Yu-Heng Chen & Wen-Chuan Lai & Ya-Fei Li & Zhi-Yuan Gu, 2025. "Native globular ferritin nanopore sensor," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    9. Fanjun Li & Monifa A. Fahie & Kaitlyn M. Gilliam & Ryan Pham & Min Chen, 2022. "Mapping the conformational energy landscape of Abl kinase using ClyA nanopore tweezers," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    10. Adam P. Miller & Steve L. Reichow, 2025. "Mechanism of small heat shock protein client sequestration and induced polydispersity," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    11. Corina N. D’Alessandro-Gabazza & Taro Yasuma & Tetsu Kobayashi & Masaaki Toda & Ahmed M. Abdel-Hamid & Hajime Fujimoto & Osamu Hataji & Hiroki Nakahara & Atsuro Takeshita & Kota Nishihama & Tomohito O, 2022. "Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis," Nature Communications, Nature, vol. 13(1), pages 1-23, December.
    12. Gašper Šolinc & Marija Srnko & Franci Merzel & Ana Crnković & Mirijam Kozorog & Marjetka Podobnik & Gregor Anderluh, 2025. "Cryo-EM structures of a protein pore reveal a cluster of cholesterol molecules and diverse roles of membrane lipids," Nature Communications, Nature, vol. 16(1), pages 1-11, December.
    13. Kübra Kaygisiz & Lena Rauch-Wirth & Arghya Dutta & Xiaoqing Yu & Yuki Nagata & Tristan Bereau & Jan Münch & Christopher V. Synatschke & Tanja Weil, 2023. "Data-mining unveils structure–property–activity correlation of viral infectivity enhancing self-assembling peptides," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    14. Ran Wang & Xianfa Yang & Jiehui Chen & Lin Zhang & Jonathan A. Griffiths & Guizhong Cui & Yingying Chen & Yun Qian & Guangdun Peng & Jinsong Li & Liantang Wang & John C. Marioni & Patrick P. L. Tam & , 2023. "Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    15. Jian Tian & Jaie C Woodard & Andrew Whitney & Eugene I Shakhnovich, 2015. "Thermal Stabilization of Dihydrofolate Reductase Using Monte Carlo Unfolding Simulations and Its Functional Consequences," PLOS Computational Biology, Public Library of Science, vol. 11(4), pages 1-27, April.
    16. Sophie Shen & Tessa Werner & Samuel W. Lukowski & Stacey Andersen & Yuliangzi Sun & Woo Jun Shim & Dalia Mizikovsky & Sakurako Kobayashi & Jennifer Outhwaite & Han Sheng Chiu & Xiaoli Chen & Gavin Cha, 2025. "Atlas of multilineage stem cell differentiation reveals TMEM88 as a developmental regulator of blood pressure," Nature Communications, Nature, vol. 16(1), pages 1-19, December.
    17. Matthias M. Schneider & Saurabh Gautam & Therese W. Herling & Ewa Andrzejewska & Georg Krainer & Alyssa M. Miller & Victoria A. Trinkaus & Quentin A. E. Peter & Francesco Simone Ruggeri & Michele Vend, 2021. "The Hsc70 disaggregation machinery removes monomer units directly from α-synuclein fibril ends," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    18. Eri Chatani & Yutaro Tsuchisaka & Yuki Masuda & Roumiana Tsenkova, 2014. "Water Molecular System Dynamics Associated with Amyloidogenic Nucleation as Revealed by Real Time Near Infrared Spectroscopy and Aquaphotomics," PLOS ONE, Public Library of Science, vol. 9(7), pages 1-10, July.
    19. Jianxin Yang & Tianle Pan & Zhenming Xie & Wu Yuan & Ho-Pui Ho, 2024. "In-tube micro-pyramidal silicon nanopore for inertial-kinetic sensing of single molecules," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    20. Ki-Baek Jeong & Minju Ryu & Jin-Sik Kim & Minsoo Kim & Jejoong Yoo & Minji Chung & Sohee Oh & Gyunghee Jo & Seong-Gyu Lee & Ho Min Kim & Mi-Kyung Lee & Seung-Wook Chi, 2023. "Single-molecule fingerprinting of protein-drug interaction using a funneled biological nanopore," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    21. repec:plo:pone00:0065358 is not listed on IDEAS
    22. Mookyung Cheon & Iksoo Chang & Sandipan Mohanty & Leila M Luheshi & Christopher M Dobson & Michele Vendruscolo & Giorgio Favrin, 2007. "Structural Reorganisation and Potential Toxicity of Oligomeric Species Formed during the Assembly of Amyloid Fibrils," PLOS Computational Biology, Public Library of Science, vol. 3(9), pages 1-12, September.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-58509-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.