IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-57980-7.html
   My bibliography  Save this article

Global characterization of mouse testis O-glycoproteome landscape during spermatogenesis

Author

Listed:
  • Qiannan Liu

    (Shanghai Jiao Tong University)

  • Xiaoyan Lu

    (Shanghai Jiao Tong University)

  • Yao Deng

    (Shanghai Jiao Tong University)

  • Han Zhang

    (Shanghai Jiao Tong University)

  • Rumeng Wei

    (Shanghai Jiao Tong University)

  • Hongrui Li

    (Shanghai Jiao Tong University)

  • Ying Feng

    (Sichuan University)

  • Juan Wei

    (Shanghai Jiao Tong University)

  • Fang Ma

    (Sichuan University)

  • Yan Zhang

    (Shanghai Jiao Tong University)

  • Xia Zou

    (Shanghai Jiao Tong University)

Abstract

Protein O-glycosylation plays critical roles in sperm formation and maturation. However, detailed knowledge on the mechanisms involved is limited due to lacking characterization of O-glycoproteome of testicular germ cells. Here, we performed a systematic analysis of site-specific O-glycosylation in mouse testis, and established an O-glycoproteome map with 349 O-glycoproteins and 799 unambiguous O-glycosites. Moreover, we comprehensively investigated the distribution properties of O-glycosylation in testis and identified a region near the N-terminal of peptidase S1 domain that is susceptible to O-glycosylation. Interestingly, we found dynamic changes with an increase Tn and a decrease T structure from early to mature developmental stages. Notably, the importance of O-glycosylation was supported by its effects on the stability, cleavage, and interaction of acrosomal proteins. Collectively, these data illustrate the global properties of O-glycosylation in testis, providing insights and resources for future functional studies targeting O-glycosylation dysregulation in male infertility.

Suggested Citation

  • Qiannan Liu & Xiaoyan Lu & Yao Deng & Han Zhang & Rumeng Wei & Hongrui Li & Ying Feng & Juan Wei & Fang Ma & Yan Zhang & Xia Zou, 2025. "Global characterization of mouse testis O-glycoproteome landscape during spermatogenesis," Nature Communications, Nature, vol. 16(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57980-7
    DOI: 10.1038/s41467-025-57980-7
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-57980-7
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-57980-7?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Siyuan Kong & Pengyun Gong & Wen-Feng Zeng & Biyun Jiang & Xinhang Hou & Yang Zhang & Huanhuan Zhao & Mingqi Liu & Guoquan Yan & Xinwen Zhou & Xihua Qiao & Mengxi Wu & Pengyuan Yang & Chao Liu & Weiqi, 2022. "pGlycoQuant with a deep residual network for quantitative glycoproteomics at intact glycopeptide level," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Thomas D. Madsen & Lasse H. Hansen & John Hintze & Zilu Ye & Shifa Jebari & Daniel B. Andersen & Hiren J. Joshi & Tongzhong Ju & Jens P. Goetze & Cesar Martin & Mette M. Rosenkilde & Jens J. Holst & R, 2020. "An atlas of O-linked glycosylation on peptide hormones reveals diverse biological roles," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Christian T. Madsen & Jan C. Refsgaard & Felix G. Teufel & Sonny K. Kjærulff & Zhe Wang & Guangjun Meng & Carsten Jessen & Petteri Heljo & Qunfeng Jiang & Xin Zhao & Bo Wu & Xueping Zhou & Yang Tang &, 2022. "Combining mass spectrometry and machine learning to discover bioactive peptides," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Weiping Sun & Qianqiu Zhang & Xiyue Zhang & Ngoc Hieu Tran & M. Ziaur Rahman & Zheng Chen & Chao Peng & Jun Ma & Ming Li & Lei Xin & Baozhen Shan, 2023. "Glycopeptide database search and de novo sequencing with PEAKS GlycanFinder enable highly sensitive glycoproteomics," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Ho Yan Yeung & Iris Bea L. Ramiro & Daniel B. Andersen & Thomas Lund Koch & Alexander Hamilton & Walden E. Bjørn-Yoshimoto & Samuel Espino & Sergey Y. Vakhrushev & Kasper B. Pedersen & Noortje Haan & , 2024. "Fish-hunting cone snail disrupts prey’s glucose homeostasis with weaponized mimetics of somatostatin and insulin," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57980-7. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.