IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-57758-x.html
   My bibliography  Save this article

MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers

Author

Listed:
  • Konstantina Georgiou

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna
    a Doctoral School of the University of Vienna and Medical University of Vienna)

  • Fatih Sarigol

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna)

  • Tobias Nimpf

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna)

  • Christian Knapp

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna
    The Barcelona Institute of Science and Technology)

  • Daria Filipczak

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna
    a Doctoral School of the University of Vienna and Medical University of Vienna)

  • Roland Foisner

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna)

  • Nana Naetar

    (Max Perutz Labs, Vienna Biocenter Campus (VBC)
    Max Perutz Labs, Medical University of Vienna)

Abstract

Spatial organization of the mammalian genome influences gene expression and cell identity. While association of genes with the nuclear periphery is commonly linked to transcriptional repression, also active, expressed genes can localize at the nuclear periphery. The transcriptionally active MyoD1 gene, a master regulator of myogenesis, exhibits peripheral localization in proliferating myoblasts, yet the underlying mechanisms remain elusive. Here, we generate a reporter cell line to demonstrate that peripheral association of the MyoD1 locus is independent of mechanisms involved in heterochromatin anchoring. Instead, we identify the nuclear envelope transmembrane protein WFS1 that tethers MyoD1 to the nuclear periphery. WFS1 primarily associates with active distal enhancer elements upstream of MyoD1, and with a subset of enhancers genome-wide, which are enriched in active histone marks and linked to expressed myogenic genes. Overall, our data identify a mechanism involved in tethering regulatory elements of active genes to the nuclear periphery.

Suggested Citation

  • Konstantina Georgiou & Fatih Sarigol & Tobias Nimpf & Christian Knapp & Daria Filipczak & Roland Foisner & Nana Naetar, 2025. "MyoD1 localization at the nuclear periphery is mediated by association of WFS1 with active enhancers," Nature Communications, Nature, vol. 16(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57758-x
    DOI: 10.1038/s41467-025-57758-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-57758-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-57758-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Lars Guelen & Ludo Pagie & Emilie Brasset & Wouter Meuleman & Marius B. Faza & Wendy Talhout & Bert H. Eussen & Annelies de Klein & Lodewyk Wessels & Wouter de Laat & Bas van Steensel, 2008. "Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions," Nature, Nature, vol. 453(7197), pages 948-951, June.
    2. K. L. Reddy & J. M. Zullo & E. Bertolino & H. Singh, 2008. "Transcriptional repression mediated by repositioning of genes to the nuclear lamina," Nature, Nature, vol. 452(7184), pages 243-247, March.
    3. Linlin Wang & Hongyang Liu & Xiaofei Zhang & Eli Song & You Wang & Tao Xu & Zonghong Li, 2021. "WFS1 functions in ER export of vesicular cargo proteins in pancreatic β-cells," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Carlos Díaz-Castillo, 2013. "Females and Males Contribute in Opposite Ways to the Evolution of Gene Order in Drosophila," PLOS ONE, Public Library of Science, vol. 8(5), pages 1-10, May.
    2. Cristiana Bersaglieri & Jelena Kresoja-Rakic & Shivani Gupta & Dominik Bär & Rostyslav Kuzyakiv & Martina Panatta & Raffaella Santoro, 2022. "Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    3. Yi Li & James Lee & Lu Bai, 2024. "DNA methylation-based high-resolution mapping of long-distance chromosomal interactions in nucleosome-depleted regions," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    4. Peter Henneman & Arjan Bouman & Adri Mul & Lia Knegt & Anne-Marie van der Kevie-Kersemaekers & Nitash Zwaveling-Soonawala & Hanne E J Meijers-Heijboer & A S Paul van Trotsenburg & Marcel M Mannens, 2018. "Widespread domain-like perturbations of DNA methylation in whole blood of Down syndrome neonates," PLOS ONE, Public Library of Science, vol. 13(3), pages 1-19, March.
    5. Frederic Li Mow Chee & Bruno Beernaert & Billie G. C. Griffith & Alexander E. P. Loftus & Yatendra Kumar & Jimi C. Wills & Martin Lee & Jessica Valli & Ann P. Wheeler & J. Douglas Armstrong & Maddy Pa, 2023. "Mena regulates nesprin-2 to control actin–nuclear lamina associations, trans-nuclear membrane signalling and gene expression," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    6. Meng Zhang & Mary Elisabeth Ehmann & Srija Matukumalli & Aashutosh Girish Boob & David M. Gilbert & Huimin Zhao, 2023. "SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    7. Timothy A. Daugird & Yu Shi & Katie L. Holland & Hosein Rostamian & Zhe Liu & Luke D. Lavis & Joseph Rodriguez & Brian D. Strahl & Wesley R. Legant, 2024. "Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    8. Miguel M. Álvarez & Josep Biayna & Fran Supek, 2022. "TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    9. Wei Jin & Shaoshuai Jiang & Xinyi Liu & Yi He & Tuo Li & Jingchun Ma & Zhihong Chen & Xiaomei Lu & Xinguang Liu & Weinian Shou & Guoxiang Jin & Junjun Ding & Zhongjun Zhou, 2024. "Disorganized chromatin hierarchy and stem cell aging in a male patient of atypical laminopathy-based progeria mandibuloacral dysplasia type A," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    10. Allison P. Siegenfeld & Shelby A. Roseman & Heejin Roh & Nicholas Z. Lue & Corin C. Wagen & Eric Zhou & Sarah E. Johnstone & Martin J. Aryee & Brian B. Liau, 2022. "Polycomb-lamina antagonism partitions heterochromatin at the nuclear periphery," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    11. David E. Torres & H. Martin Kramer & Vittorio Tracanna & Gabriel L. Fiorin & David E. Cook & Michael F. Seidl & Bart P. H. J. Thomma, 2024. "Implications of the three-dimensional chromatin organization for genome evolution in a fungal plant pathogen," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    12. Robert Schöpflin & Uirá Souto Melo & Hossein Moeinzadeh & David Heller & Verena Laupert & Jakob Hertzberg & Manuel Holtgrewe & Nico Alavi & Marius-Konstantin Klever & Julius Jungnitsch & Emel Comak & , 2022. "Integration of Hi-C with short and long-read genome sequencing reveals the structure of germline rearranged genomes," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    13. Yinuo Wang & Adel Elsherbiny & Linda Kessler & Julio Cordero & Haojie Shi & Heike Serke & Olga Lityagina & Felix A. Trogisch & Mona Malek Mohammadi & Ibrahim El-Battrawy & Johannes Backs & Thomas Wiel, 2022. "Lamin A/C-dependent chromatin architecture safeguards naïve pluripotency to prevent aberrant cardiovascular cell fate and function," Nature Communications, Nature, vol. 13(1), pages 1-24, December.
    14. Kaela M. Varberg & Esteban M. Dominguez & Boryana Koseva & Joseph M. Varberg & Ross P. McNally & Ayelen Moreno-Irusta & Emily R. Wesley & Khursheed Iqbal & Warren A. Cheung & Carl Schwendinger-Schreck, 2023. "Extravillous trophoblast cell lineage development is associated with active remodeling of the chromatin landscape," Nature Communications, Nature, vol. 14(1), pages 1-23, December.
    15. Judith H. I. Haarhuis & Robin H. Weide & Vincent A. Blomen & Koen D. Flach & Hans Teunissen & Laureen Willems & Thijn R. Brummelkamp & Benjamin D. Rowland & Elzo Wit, 2022. "A Mediator-cohesin axis controls heterochromatin domain formation," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57758-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.