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Multi-scale dynamics influence the division potential of stomatal lineage ground cells in Arabidopsis

Author

Listed:
  • Hannah F. Fung

    (Stanford University
    Howard Hughes Medical Institute)

  • Gabriel O. Amador

    (Stanford University)

  • Renee Dale

    (Donald Danforth Plant Science Center)

  • Yan Gong

    (Stanford University
    Harvard University)

  • Macy Vollbrecht

    (Stanford University)

  • Joel M. Erberich

    (Stanford University)

  • Andrea Mair

    (Howard Hughes Medical Institute)

  • Dominique C. Bergmann

    (Stanford University
    Howard Hughes Medical Institute)

Abstract

During development, many precursor lineages are flexible, producing variable numbers and types of progeny cells. What determines whether precursors differentiate or continue dividing? Here we take a quantitative approach that combines long-term live imaging, statistical modeling and computational simulations to probe the developmental flexibility of stomatal lineage ground cells (SLGC) in Arabidopsis leaves. We discover that cell size is a strong predictor of SLGC behaviour and that cell size is linked to division behaviour at multiple spatial scales. At the neighbourhood scale, cell size correlates with the strength of cell-cell signaling, which affects the rate at which SPEECHLESS (SPCH), a division-promoting transcription factor, is degraded. At the subcellular scale, cell size correlates with nuclear size, which modulates the concentration of SPCH in the nucleus. Our work shows how initial differences in SPCH levels are canalized by nuclear size and cell-cell signaling to inform the behaviour of a flexible cell type.

Suggested Citation

  • Hannah F. Fung & Gabriel O. Amador & Renee Dale & Yan Gong & Macy Vollbrecht & Joel M. Erberich & Andrea Mair & Dominique C. Bergmann, 2025. "Multi-scale dynamics influence the division potential of stomatal lineage ground cells in Arabidopsis," Nature Communications, Nature, vol. 16(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57730-9
    DOI: 10.1038/s41467-025-57730-9
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    References listed on IDEAS

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    1. Benjamin R. Doughty & Michaela M. Hinks & Julia M. Schaepe & Georgi K. Marinov & Abby R. Thurm & Carolina Rios-Martinez & Benjamin E. Parks & Yingxuan Tan & Emil Marklund & Danilo Dubocanin & Lacramio, 2024. "Single-molecule states link transcription factor binding to gene expression," Nature, Nature, vol. 636(8043), pages 745-754, December.
    2. Cara M. Winter & Pablo Szekely & Vladimir Popov & Heather Belcher & Raina Carter & Matthew Jones & Scott E. Fraser & Thai V. Truong & Philip N. Benfey, 2024. "SHR and SCR coordinate root patterning and growth early in the cell cycle," Nature, Nature, vol. 626(7999), pages 611-616, February.
    3. Anaxi Houbaert & Cheng Zhang & Manish Tiwari & Kun Wang & Alberto Marcos Serrano & Daniel V. Savatin & Mounashree J. Urs & Miroslava K. Zhiponova & Gustavo E. Gudesblat & Isabelle Vanhoutte & Dominiqu, 2018. "POLAR-guided signalling complex assembly and localization drive asymmetric cell division," Nature, Nature, vol. 563(7732), pages 574-578, November.
    4. Jonathan Fiorentino & Antonio Scialdone, 2022. "The role of cell geometry and cell-cell communication in gradient sensing," PLOS Computational Biology, Public Library of Science, vol. 18(3), pages 1-22, March.
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