IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-025-57458-6.html
   My bibliography  Save this article

Histidine 73 methylation coordinates β-actin plasticity in response to key environmental factors

Author

Listed:
  • Adrien Schahl

    (Université de Toulouse, CNRS
    Sorbonne Université, UMR 7616 CNRS)

  • Louis Lagardère

    (Sorbonne Université, UMR 7616 CNRS)

  • Brandon Walker

    (The University of Texas at Austin)

  • Pengyu Ren

    (The University of Texas at Austin)

  • Hugo Wioland

    (Université Paris Cité, CNRS)

  • Maya Ballet

    (Université Paris Cité, CNRS)

  • Antoine Jégou

    (Université Paris Cité, CNRS)

  • Matthieu Chavent

    (Université de Toulouse, CNRS
    Université de Toulouse, CNRS)

  • Jean-Philip Piquemal

    (Sorbonne Université, UMR 7616 CNRS)

Abstract

The functional importance of the methylation of histidine 73 (H73) in actin remains unclear. Focusing on cytoplasmic β-actin, present in all mammalian cells, we use molecular dynamics simulations with a polarizable force field and adaptive sampling to examine the effects of H73 methylation. Our results show that methylation enhances nucleotide binding cleft opening, alters allosteric pathways connecting subdomains 2 and 4 (SD2 and SD4) in G-actin, and affects backdoor openings and inorganic phosphate release in F-actin, as validated by biochemical assays. These effects depend on the nucleotide and ions interacting with the actin. Together, our findings reveal how H73 methylation regulates β-actin plasticity and integrates environmental cues.

Suggested Citation

  • Adrien Schahl & Louis Lagardère & Brandon Walker & Pengyu Ren & Hugo Wioland & Maya Ballet & Antoine Jégou & Matthieu Chavent & Jean-Philip Piquemal, 2025. "Histidine 73 methylation coordinates β-actin plasticity in response to key environmental factors," Nature Communications, Nature, vol. 16(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57458-6
    DOI: 10.1038/s41467-025-57458-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-025-57458-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-025-57458-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57458-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.