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Heliorhodopsin-mediated light-modulation of ABC transporter

Author

Listed:
  • Shin-Gyu Cho

    (Sogang University
    Sogang University)

  • Ji-Hyun Kim

    (Sogang University)

  • Ji-eun Lee

    (Sogang University)

  • In-Jung Choi

    (Sogang University)

  • Myungchul Song

    (Sogang University)

  • Kimleng Chuon

    (Sogang University)

  • Jin-gon Shim

    (Sogang University)

  • Kun-Wook Kang

    (Sogang University)

  • Kwang-Hwan Jung

    (Sogang University)

Abstract

Heliorhodopsins (HeRs) have been hypothesized to have widespread functions. Recently, the functions for few HeRs have been revealed; however, the hypothetical functions remain largely unknown. Herein, we investigate light-modulation of heterodimeric multidrug resistance ATP-binding cassette transporters (OmrDE) mediated by Omithinimicrobium cerasi HeR. In this study, we classifiy genes flanking the HeR-encoding genes and identify highly conservative residues for protein–protein interactions. Our results reveal that the interaction between OcHeR and OmrDE shows positive cooperatively sequential binding through thermodynamic parameters. Moreover, light-induced OcHeR upregulates OmrDE drug transportation. Hence, the binding may be crucial to drug resistance in O. cerasi as it survives in a drug-containing habitat. Overall, we unveil a function of HeR as regulatory rhodopsin for multidrug resistance. Our findings suggest potential applications in optogenetic technology.

Suggested Citation

  • Shin-Gyu Cho & Ji-Hyun Kim & Ji-eun Lee & In-Jung Choi & Myungchul Song & Kimleng Chuon & Jin-gon Shim & Kun-Wook Kang & Kwang-Hwan Jung, 2024. "Heliorhodopsin-mediated light-modulation of ABC transporter," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48650-1
    DOI: 10.1038/s41467-024-48650-1
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    References listed on IDEAS

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    1. Wataru Shihoya & Keiichi Inoue & Manish Singh & Masae Konno & Shoko Hososhima & Keitaro Yamashita & Kento Ikeda & Akimitsu Higuchi & Tamaki Izume & Sae Okazaki & Masanori Hashimoto & Ritsu Mizutori & , 2019. "Crystal structure of heliorhodopsin," Nature, Nature, vol. 574(7776), pages 132-136, October.
    2. Alina Pushkarev & Keiichi Inoue & Shirley Larom & José Flores-Uribe & Manish Singh & Masae Konno & Sahoko Tomida & Shota Ito & Ryoko Nakamura & Satoshi P. Tsunoda & Alon Philosof & Itai Sharon & Natal, 2018. "A distinct abundant group of microbial rhodopsins discovered using functional metagenomics," Nature, Nature, vol. 558(7711), pages 595-599, June.
    3. Susanne Hofmann & Dovile Januliene & Ahmad R. Mehdipour & Christoph Thomas & Erich Stefan & Stefan Brüchert & Benedikt T. Kuhn & Eric R. Geertsma & Gerhard Hummer & Robert Tampé & Arne Moeller, 2019. "Conformation space of a heterodimeric ABC exporter under turnover conditions," Nature, Nature, vol. 571(7766), pages 580-583, July.
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