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Structural basis of Acinetobacter type IV pili targeting by an RNA virus

Author

Listed:
  • Ran Meng

    (Texas A&M University
    Yale University)

  • Zhongliang Xing

    (Texas A&M University)

  • Jeng-Yih Chang

    (Texas A&M University
    UMass Chan Medical School)

  • Zihao Yu

    (Texas A&M University)

  • Jirapat Thongchol

    (Texas A&M University)

  • Wen Xiao

    (Texas A&M University)

  • Yuhang Wang

    (Texas A&M University)

  • Karthik Chamakura

    (Texas A&M University
    Inc.)

  • Zhiqi Zeng

    (Texas A&M University)

  • Fengbin Wang

    (University of Alabama at Birmingham)

  • Ry Young

    (Texas A&M University)

  • Lanying Zeng

    (Texas A&M University)

  • Junjie Zhang

    (Texas A&M University)

Abstract

Acinetobacters pose a significant threat to human health, especially those with weakened immune systems. Type IV pili of acinetobacters play crucial roles in virulence and antibiotic resistance. Single-stranded RNA bacteriophages target the bacterial retractile pili, including type IV. Our study delves into the interaction between Acinetobacter phage AP205 and type IV pili. Using cryo-electron microscopy, we solve structures of the AP205 virion with an asymmetric dimer of maturation proteins, the native Acinetobacter type IV pili bearing a distinct post-translational pilin cleavage, and the pili-bound AP205 showing its maturation proteins adapted to pilin modifications, allowing each phage to bind to one or two pili. Leveraging these results, we develop a 20-kilodalton AP205-derived protein scaffold targeting type IV pili in situ, with potential for research and diagnostics.

Suggested Citation

  • Ran Meng & Zhongliang Xing & Jeng-Yih Chang & Zihao Yu & Jirapat Thongchol & Wen Xiao & Yuhang Wang & Karthik Chamakura & Zhiqi Zeng & Fengbin Wang & Ry Young & Lanying Zeng & Junjie Zhang, 2024. "Structural basis of Acinetobacter type IV pili targeting by an RNA virus," Nature Communications, Nature, vol. 15(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47119-5
    DOI: 10.1038/s41467-024-47119-5
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    References listed on IDEAS

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    1. Karthik R. Chamakura & Jennifer S. Tran & Chandler O’Leary & Hannah G. Lisciandro & Sophia F. Antillon & Kameron D. Garza & Elizabeth Tran & Lorna Min & Ry Young, 2020. "Rapid de novo evolution of lysis genes in single-stranded RNA phages," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    2. Ran Meng & Mengqiu Jiang & Zhicheng Cui & Jeng-Yih Chang & Kailu Yang & Joanita Jakana & Xinzhe Yu & Zhao Wang & Bo Hu & Junjie Zhang, 2019. "Structural basis for the adsorption of a single-stranded RNA bacteriophage," Nature Communications, Nature, vol. 10(1), pages 1-8, December.
    3. Xinghong Dai & Zhihai Li & Mason Lai & Sara Shu & Yushen Du & Z. Hong Zhou & Ren Sun, 2017. "In situ structures of the genome and genome-delivery apparatus in a single-stranded RNA virus," Nature, Nature, vol. 541(7635), pages 112-116, January.
    4. Roman I Koning & Josue Gomez-Blanco & Inara Akopjana & Javier Vargas & Andris Kazaks & Kaspars Tars & José María Carazo & Abraham J. Koster, 2016. "Asymmetric cryo-EM reconstruction of phage MS2 reveals genome structure in situ," Nature Communications, Nature, vol. 7(1), pages 1-6, November.
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