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Projective light-sheet microscopy with flexible parameter selection

Author

Listed:
  • Bingying Chen

    (University of Texas Southwestern Medical Center)

  • Bo-Jui Chang

    (University of Texas Southwestern Medical Center)

  • Stephan Daetwyler

    (University of Texas Southwestern Medical Center)

  • Felix Zhou

    (University of Texas Southwestern Medical Center)

  • Shiv Sharma

    (University of Texas Southwestern Medical Center)

  • Donghoon M. Lee

    (University of Texas Southwestern Medical Center)

  • Amruta Nayak

    (University of Chicago)

  • Jungsik Noh

    (University of Texas Southwestern Medical Center)

  • Konstantin Dubrovinski

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

  • Elizabeth H. Chen

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

  • Michael Glotzer

    (University of Chicago)

  • Reto Fiolka

    (University of Texas Southwestern Medical Center
    University of Texas Southwestern Medical Center)

Abstract

Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid projective light-sheet imaging with parameter selection (props) of imaging depth, position and viewing angle. This allows us to selectively image different sub-volumes of a sample, rapidly switch between them and exclude background fluorescence. Here we demonstrate the power of props by functional imaging within distinct regions of the zebrafish brain, monitoring calcium firing inside muscle cells of moving Drosophila larvae, super-resolution imaging of selected cell layers, and by optically unwrapping the curved surface of a Drosophila embryo. We anticipate that props will accelerate volumetric interrogation, ranging from subcellular to mesoscopic scales.

Suggested Citation

  • Bingying Chen & Bo-Jui Chang & Stephan Daetwyler & Felix Zhou & Shiv Sharma & Donghoon M. Lee & Amruta Nayak & Jungsik Noh & Konstantin Dubrovinski & Elizabeth H. Chen & Michael Glotzer & Reto Fiolka, 2024. "Projective light-sheet microscopy with flexible parameter selection," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46693-y
    DOI: 10.1038/s41467-024-46693-y
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    References listed on IDEAS

    as
    1. Benjamin Schmid & Gopi Shah & Nico Scherf & Michael Weber & Konstantin Thierbach & Citlali Pérez Campos & Ingo Roeder & Pia Aanstad & Jan Huisken, 2013. "High-speed panoramic light-sheet microscopy reveals global endodermal cell dynamics," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
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