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Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex

Author

Listed:
  • Christina Koupourtidou

    (LMU Munich
    LMU Munich)

  • Veronika Schwarz

    (LMU Munich
    LMU Munich)

  • Hananeh Aliee

    (Helmholtz Zentrum München-German Research Center for Environmental Health)

  • Simon Frerich

    (LMU Munich
    LMU Munich)

  • Judith Fischer-Sternjak

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health)

  • Riccardo Bocchi

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health
    University of Geneva)

  • Tatiana Simon-Ebert

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health)

  • Xianshu Bai

    (University of Saarland
    University of Saarland)

  • Swetlana Sirko

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health)

  • Frank Kirchhoff

    (University of Saarland
    University of Saarland
    University of Medicine and Pharmacy of Craiova)

  • Martin Dichgans

    (LMU Munich
    LMU Munich
    German Centre for Neurodegenerative Diseases)

  • Magdalena Götz

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health
    LMU Munich)

  • Fabian J. Theis

    (Helmholtz Zentrum München-German Research Center for Environmental Health
    Technical University of Munich)

  • Jovica Ninkovic

    (LMU Munich
    Helmholtz Zentrum München-German Research Center for Environmental Health
    LMU Munich)

Abstract

Traumatic brain injury leads to a highly orchestrated immune- and glial cell response partially responsible for long-lasting disability and the development of secondary neurodegenerative diseases. A holistic understanding of the mechanisms controlling the responses of specific cell types and their crosstalk is required to develop an efficient strategy for better regeneration. Here, we combine spatial and single-cell transcriptomics to chart the transcriptomic signature of the injured male murine cerebral cortex, and identify specific states of different glial cells contributing to this signature. Interestingly, distinct glial cells share a large fraction of injury-regulated genes, including inflammatory programs downstream of the innate immune-associated pathways Cxcr3 and Tlr1/2. Systemic manipulation of these pathways decreases the reactivity state of glial cells associated with poor regeneration. The functional relevance of the discovered shared signature of glial cells highlights the importance of our resource enabling comprehensive analysis of early events after brain injury.

Suggested Citation

  • Christina Koupourtidou & Veronika Schwarz & Hananeh Aliee & Simon Frerich & Judith Fischer-Sternjak & Riccardo Bocchi & Tatiana Simon-Ebert & Xianshu Bai & Swetlana Sirko & Frank Kirchhoff & Martin Di, 2024. "Shared inflammatory glial cell signature after stab wound injury, revealed by spatial, temporal, and cell-type-specific profiling of the murine cerebral cortex," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46625-w
    DOI: 10.1038/s41467-024-46625-w
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    References listed on IDEAS

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