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Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models

Author

Listed:
  • Jia Cao

    (Central South University
    Hunan Key Laboratory of Angmedicine
    Central South University)

  • Ling Jin

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Zi-Qi Yan

    (Central South University)

  • Xiao-Kai Wang

    (Central South University)

  • You-You Li

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Zun Wang

    (Central South University)

  • Yi-Wei Liu

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Hong-Ming Li

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Zhe Guan

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Ze-Hui He

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Jiang-Shan Gong

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Jiang-Hua Liu

    (Central South University)

  • Hao Yin

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Yi-Juan Tan

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Chun-Gu Hong

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Shi-Kai Feng

    (Central South University)

  • Yan Zhang

    (Central South University)

  • Yi-Yi Wang

    (Central South University
    Hunan Key Laboratory of Angmedicine)

  • Lu-Yue Qi

    (Central South University)

  • Chun-Yuan Chen

    (Central South University
    Hunan Key Laboratory of Angmedicine
    Central South University)

  • Zheng-Zhao Liu

    (Central South University
    Hunan Key Laboratory of Angmedicine
    Central South University)

  • Zhen-Xing Wang

    (Central South University
    Hunan Key Laboratory of Angmedicine
    Central South University)

  • Hui Xie

    (Central South University
    Hunan Key Laboratory of Angmedicine
    Central South University)

Abstract

Endothelial cells (ECs) and bone marrow stromal cells (BMSCs) play crucial roles in supporting hematopoiesis and hematopoietic regeneration. However, whether ECs are a source of BMSCs remains unclear. Here, we evaluate the contribution of endothelial-to-mesenchymal transition to BMSC generation in postnatal mice. Single-cell RNA sequencing identifies ECs expressing BMSC markers Prrx1 and Lepr; however, this could not be validated using Prrx1-Cre and Lepr-Cre transgenic mice. Additionally, only a minority of BMSCs are marked by EC lineage tracing models using Cdh5-rtTA-tetO-Cre or Tek-CreERT2. Moreover, Cdh5+ BMSCs and Tek+ BMSCs show distinct spatial distributions and characteristic mesenchymal markers, suggestive of their origination from different progenitors rather than CDH5+ TEK+ ECs. Furthermore, myeloablation induced by 5-fluorouracil treatment does not increase Cdh5+ BMSCs. Our findings indicate that ECs hardly convert to BMSCs during homeostasis and myeloablation-induced hematopoietic regeneration, highlighting the importance of using appropriate genetic models and conducting careful data interpretation in studies concerning endothelial-to-mesenchymal transition.

Suggested Citation

  • Jia Cao & Ling Jin & Zi-Qi Yan & Xiao-Kai Wang & You-You Li & Zun Wang & Yi-Wei Liu & Hong-Ming Li & Zhe Guan & Ze-Hui He & Jiang-Shan Gong & Jiang-Hua Liu & Hao Yin & Yi-Juan Tan & Chun-Gu Hong & Shi, 2023. "Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-44312-w
    DOI: 10.1038/s41467-023-44312-w
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    References listed on IDEAS

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