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Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach

Author

Listed:
  • Mateusz Noszka

    (Polish Academy of Sciences)

  • Agnieszka Strzałka

    (University of Wrocław)

  • Jakub Muraszko

    (Polish Academy of Sciences)

  • Rafał Kolenda

    (Wrocław University of Environmental and Life Sciences
    Quadram Institute Biosciences, Norwich Research Park)

  • Chen Meng

    (Technical University of Munich (TUM))

  • Christina Ludwig

    (Technical University of Munich (TUM))

  • Kerstin Stingl

    (German Federal Institute for Risk Assessment)

  • Anna Zawilak-Pawlik

    (Polish Academy of Sciences)

Abstract

The gastric human pathogen Helicobacter pylori has developed mechanisms to combat stress factors, including reactive oxygen species (ROS). Here, we present a comprehensive study on the redox switch protein HP1021 regulon combining transcriptomic, proteomic and DNA-protein interactions analyses. Our results indicate that HP1021 modulates H. pylori’s response to oxidative stress. HP1021 controls the transcription of 497 genes, including 407 genes related to response to oxidative stress. 79 proteins are differently expressed in the HP1021 deletion mutant. HP1021 controls typical ROS response pathways (katA, rocF) and less canonical ones, particularly DNA uptake and central carbohydrate metabolism. HP1021 is a molecular regulator of competence in H. pylori, as HP1021-dependent repression of the comB DNA uptake genes is relieved under oxidative conditions, increasing natural competence. Furthermore, HP1021 controls glucose consumption by directly regulating the gluP transporter and has an important impact on maintaining the energetic balance in the cell.

Suggested Citation

  • Mateusz Noszka & Agnieszka Strzałka & Jakub Muraszko & Rafał Kolenda & Chen Meng & Christina Ludwig & Kerstin Stingl & Anna Zawilak-Pawlik, 2023. "Profiling of the Helicobacter pylori redox switch HP1021 regulon using a multi-omics approach," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-42364-6
    DOI: 10.1038/s41467-023-42364-6
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    1. Cynthia M. Sharma & Steve Hoffmann & Fabien Darfeuille & Jérémy Reignier & Sven Findeiß & Alexandra Sittka & Sandrine Chabas & Kristin Reiche & Jörg Hackermüller & Richard Reinhardt & Peter F. Stadler, 2010. "The primary transcriptome of the major human pathogen Helicobacter pylori," Nature, Nature, vol. 464(7286), pages 250-255, March.
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