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Chemoenzymatic synthesis of genetically-encoded multivalent liquid N-glycan arrays

Author

Listed:
  • Chih-Lan Lin

    (University of Alberta)

  • Mirat Sojitra

    (University of Alberta)

  • Eric J. Carpenter

    (University of Alberta)

  • Ellen S. Hayhoe

    (University of Alberta)

  • Susmita Sarkar

    (University of Alberta)

  • Elizabeth A. Volker

    (University of Alberta)

  • Chao Wang

    (The Scripps Research Institute)

  • Duong T. Bui

    (University of Alberta)

  • Loretta Yang

    (Lectenz Bio)

  • John S. Klassen

    (University of Alberta)

  • Peng Wu

    (The Scripps Research Institute)

  • Matthew S. Macauley

    (University of Alberta
    University of Alberta)

  • Todd L. Lowary

    (University of Alberta
    Academia Sinica
    National Taiwan University)

  • Ratmir Derda

    (University of Alberta)

Abstract

Cellular glycosylation is characterized by chemical complexity and heterogeneity, which is challenging to reproduce synthetically. Here we show chemoenzymatic synthesis on phage to produce a genetically-encoded liquid glycan array (LiGA) of complex type N-glycans. Implementing the approach involved by ligating an azide-containing sialylglycosyl-asparagine to phage functionalized with 50–1000 copies of dibenzocyclooctyne. The resulting intermediate can be trimmed by glycosidases and extended by glycosyltransferases yielding a phage library with different N-glycans. Post-reaction analysis by MALDI-TOF MS allows rigorous characterization of N-glycan structure and mean density, which are both encoded in the phage DNA. Use of this LiGA with fifteen glycan-binding proteins, including CD22 or DC-SIGN on cells, reveals optimal structure/density combinations for recognition. Injection of the LiGA into mice identifies glycoconjugates with structures and avidity necessary for enrichment in specific organs. This work provides a quantitative evaluation of the interaction of complex N-glycans with GBPs in vitro and in vivo.

Suggested Citation

  • Chih-Lan Lin & Mirat Sojitra & Eric J. Carpenter & Ellen S. Hayhoe & Susmita Sarkar & Elizabeth A. Volker & Chao Wang & Duong T. Bui & Loretta Yang & John S. Klassen & Peng Wu & Matthew S. Macauley & , 2023. "Chemoenzymatic synthesis of genetically-encoded multivalent liquid N-glycan arrays," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40900-y
    DOI: 10.1038/s41467-023-40900-y
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    References listed on IDEAS

    as
    1. Jennie Grace Briard & Hao Jiang & Kelley W. Moremen & Matthew Scott Macauley & Peng Wu, 2018. "Cell-based glycan arrays for probing glycan–glycan binding protein interactions," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
    2. Edward N. Schmidt & Dimitra Lamprinaki & Kelli A. McCord & Maju Joe & Mirat Sojitra & Ayk Waldow & Jasmine Nguyen & John Monyror & Elena N. Kitova & Fahima Mozaneh & Xue Yan Guo & Jaesoo Jung & Jhon R, 2023. "Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    3. Emily Rodrigues & Jaesoo Jung & Heajin Park & Caleb Loo & Sepideh Soukhtehzari & Elena N. Kitova & Fahima Mozaneh & Gour Daskhan & Edward N. Schmidt & Vivian Aghanya & Susmita Sarkar & Laura Streith &, 2020. "A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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