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Two single-point mutations in Ankyrin Repeat one drastically change the threshold temperature of TRPV1

Author

Listed:
  • Shogo Hori

    (Nagahama Institute of Bio-Science and Technology)

  • Michihiro Tateyama

    (Division of Biophysics and Neurobiology, National Institute for Physiological Sciences)

  • Tsuyoshi Shirai

    (Nagahama Institute of Bio-Science and Technology)

  • Yoshihiro Kubo

    (Division of Biophysics and Neurobiology, National Institute for Physiological Sciences)

  • Osamu Saitoh

    (Nagahama Institute of Bio-Science and Technology
    Nagahama Institute of Bio-Science and Technology)

Abstract

TRPV1 plays an important role in the thermosensory system; however, the mechanism controlling its heat activation property is not well understood. Here, we determine the heat activation properties of TRPV1 cloned from tailed amphibians, which prefer cooler environments, finding the threshold temperatures were approximately 10 °C lower compared with rat TRPV1 (rTRPV1). We find that two amino acid residues (Gln, Leu/Val) in the Ankyrin Repeat 1 (ANK1) region of the N-terminal domain are conserved among tailed amphibians and different from those (Arg, Lys) in rTRPV1. We observe the activation by heat in all urodelan TRPV1s is markedly elevated by substitution of these two amino acids. Conversely, reciprocal substitutions of rTRPV1 apparently lowers the high threshold temperature. Our studies demonstrate that tailed amphibians express TRPV1 with a reduced heat-activation threshold by substitution of two amino acid residues in the ANK1 region that likely contribute to cool-habitat selection.

Suggested Citation

  • Shogo Hori & Michihiro Tateyama & Tsuyoshi Shirai & Yoshihiro Kubo & Osamu Saitoh, 2023. "Two single-point mutations in Ankyrin Repeat one drastically change the threshold temperature of TRPV1," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38051-1
    DOI: 10.1038/s41467-023-38051-1
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    References listed on IDEAS

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