IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37945-4.html
   My bibliography  Save this article

DNMT1 mutant ants develop normally but have disrupted oogenesis

Author

Listed:
  • Iryna Ivasyk

    (The Rockefeller University)

  • Leonora Olivos-Cisneros

    (The Rockefeller University)

  • Stephany Valdés-Rodríguez

    (The Rockefeller University
    Howard Hughes Medical Institute)

  • Marie Droual

    (The Rockefeller University)

  • Hosung Jang

    (University of Georgia)

  • Robert J. Schmitz

    (University of Georgia)

  • Daniel J. C. Kronauer

    (The Rockefeller University
    Howard Hughes Medical Institute)

Abstract

Although DNA methylation is an important gene regulatory mechanism in mammals, its function in arthropods remains poorly understood. Studies in eusocial insects have argued for its role in caste development by regulating gene expression and splicing. However, such findings are not always consistent across studies, and have therefore remained controversial. Here we use CRISPR/Cas9 to mutate the maintenance DNA methyltransferase DNMT1 in the clonal raider ant, Ooceraea biroi. Mutants have greatly reduced DNA methylation, but no obvious developmental phenotypes, demonstrating that, unlike mammals, ants can undergo normal development without DNMT1 or DNA methylation. Additionally, we find no evidence of DNA methylation regulating caste development. However, mutants are sterile, whereas in wild-type ants, DNMT1 is localized to the ovaries and maternally provisioned into nascent oocytes. This supports the idea that DNMT1 plays a crucial but unknown role in the insect germline.

Suggested Citation

  • Iryna Ivasyk & Leonora Olivos-Cisneros & Stephany Valdés-Rodríguez & Marie Droual & Hosung Jang & Robert J. Schmitz & Daniel J. C. Kronauer, 2023. "DNMT1 mutant ants develop normally but have disrupted oogenesis," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37945-4
    DOI: 10.1038/s41467-023-37945-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37945-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37945-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Matthew D. Schultz & Yupeng He & John W. Whitaker & Manoj Hariharan & Eran A. Mukamel & Danny Leung & Nisha Rajagopal & Joseph R. Nery & Mark A. Urich & Huaming Chen & Shin Lin & Yiing Lin & Inkyung J, 2015. "Human body epigenome maps reveal noncanonical DNA methylation variation," Nature, Nature, vol. 523(7559), pages 212-216, July.
    2. Sebastian Alvarado & Rajendhran Rajakumar & Ehab Abouheif & Moshe Szyf, 2015. "Epigenetic variation in the Egfr gene generates quantitative variation in a complex trait in ants," Nature Communications, Nature, vol. 6(1), pages 1-9, May.
    3. Serafino Teseo & Nicolas Châline & Pierre Jaisson & Daniel J.C. Kronauer, 2014. "Epistasis between adults and larvae underlies caste fate and fitness in a clonal ant," Nature Communications, Nature, vol. 5(1), pages 1-8, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Qiang Zhang & Sai Ma & Zhengzhi Liu & Bohan Zhu & Zirui Zhou & Gaoshan Li & J. Javier Meana & Javier González-Maeso & Chang Lu, 2023. "Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomes," Nature Communications, Nature, vol. 14(1), pages 1-10, December.
    2. Xiaomeng Qiao & Fangyuan Yin & Yuanyuan Ji & Yunxiao Li & Peng Yan & Jianghua Lai, 2017. "5-Aza-2’-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats," PLOS ONE, Public Library of Science, vol. 12(6), pages 1-17, June.
    3. Andrea L Liebl & Jeff S Wesner & Andrew F Russell & Aaron W Schrey, 2021. "Methylation patterns at fledging predict delayed dispersal in a cooperatively breeding bird," PLOS ONE, Public Library of Science, vol. 16(6), pages 1-13, June.
    4. Jamie L. Endicott & Paula A. Nolte & Hui Shen & Peter W. Laird, 2022. "Cell division drives DNA methylation loss in late-replicating domains in primary human cells," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    5. Yinwen Zhang & Hosung Jang & Rui Xiao & Ioanna Kakoulidou & Robert S. Piecyk & Frank Johannes & Robert J. Schmitz, 2021. "Heterochromatin is a quantitative trait associated with spontaneous epiallele formation," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    6. Hagar Taman & Christopher G Fenton & Endre Anderssen & Jon Florholmen & Ruth H Paulssen, 2021. "DNA hypo-methylation facilitates anti-inflammatory responses in severe ulcerative colitis," PLOS ONE, Public Library of Science, vol. 16(4), pages 1-17, April.
    7. Liam McAllan & Damir Baranasic & Sergio Villicaña & Scarlett Brown & Weihua Zhang & Benjamin Lehne & Marco Adamo & Andrew Jenkinson & Mohamed Elkalaawy & Borzoueh Mohammadi & Majid Hashemi & Nadia Fer, 2023. "Integrative genomic analyses in adipocytes implicate DNA methylation in human obesity and diabetes," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    8. Brittany Kraft & Valerie A Lemakos & Joseph Travis & Kimberly A Hughes & John FitzpatrickHandling editor, 2018. "Pervasive indirect genetic effects on behavioral development in polymorphic eastern mosquitofish," Behavioral Ecology, International Society for Behavioral Ecology, vol. 29(2), pages 289-300.
    9. Patrick K Piekarski & Stephany Valdés-Rodríguez & Daniel J C Kronauer, 2023. "Conditional indirect genetic effects of caregivers on brood in the clonal raider ant," Behavioral Ecology, International Society for Behavioral Ecology, vol. 34(4), pages 642-652.
    10. Johanna Klughammer & Daria Romanovskaia & Amelie Nemc & Annika Posautz & Charlotte A. Seid & Linda C. Schuster & Melissa C. Keinath & Juan Sebastian Lugo Ramos & Lindsay Kosack & Ann Evankow & Dieter , 2023. "Comparative analysis of genome-scale, base-resolution DNA methylation profiles across 580 animal species," Nature Communications, Nature, vol. 14(1), pages 1-23, December.
    11. Victoria L. Sork & Shawn J. Cokus & Sorel T. Fitz-Gibbon & Aleksey V. Zimin & Daniela Puiu & Jesse A. Garcia & Paul F. Gugger & Claudia L. Henriquez & Ying Zhen & Kirk E. Lohmueller & Matteo Pellegrin, 2022. "High-quality genome and methylomes illustrate features underlying evolutionary success of oaks," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    12. Min-Rui-Xuan Xu & Zhen-Yang Liao & Jordan R. Brock & Kang Du & Guo-Yin Li & Zhi-Qiang Chen & Ying-Hao Wang & Zhong-Nan Gao & Gaurav Agarwal & Kevin H-C Wei & Feng Shao & Shuai Pang & Adrian E. Platts , 2023. "Maternal dominance contributes to subgenome differentiation in allopolyploid fishes," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37945-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.