IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37911-0.html
   My bibliography  Save this article

Influenza virus mRNAs encode determinants for nuclear export via the cellular TREX-2 complex

Author

Listed:
  • Prasanna Bhat

    (University of Texas Southwestern Medical Center)

  • Vasilisa Aksenova

    (National Institutes of Health)

  • Matthew Gazzara

    (University of Pennsylvania)

  • Emily A. Rex

    (University of Texas Southwestern Medical Center)

  • Sadaf Aslam

    (Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai)

  • Christina Haddad

    (Case Western Reserve University)

  • Shengyan Gao

    (University of Texas Southwestern Medical Center)

  • Matthew Esparza

    (University of Texas Southwestern Medical Center)

  • Tolga Cagatay

    (University of Texas Southwestern Medical Center)

  • Kimberly Batten

    (University of Texas Southwestern Medical Center)

  • Sara S. El Zahed

    (Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai)

  • Alexei Arnaoutov

    (National Institutes of Health)

  • Hualin Zhong

    (Hunter College)

  • Jerry W. Shay

    (University of Texas Southwestern Medical Center)

  • Blanton S. Tolbert

    (Case Western Reserve University)

  • Mary Dasso

    (National Institutes of Health)

  • Kristen W. Lynch

    (University of Pennsylvania)

  • Adolfo García-Sastre

    (Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai
    Icahn School of Medicine at Mount Sinai)

  • Beatriz M. A. Fontoura

    (University of Texas Southwestern Medical Center)

Abstract

Nuclear export of influenza A virus (IAV) mRNAs occurs through the nuclear pore complex (NPC). Using the Auxin-Induced Degron (AID) system to rapidly degrade proteins, we show that among the nucleoporins localized at the nucleoplasmic side of the NPC, TPR is the key nucleoporin required for nuclear export of influenza virus mRNAs. TPR recruits the TRanscription and EXport complex (TREX)−2 to the NPC for exporting a subset of cellular mRNAs. By degrading components of the TREX-2 complex (GANP, Germinal-center Associated Nuclear Protein; PCID2, PCI domain containing 2), we show that influenza mRNAs require the TREX-2 complex for nuclear export and replication. Furthermore, we found that cellular mRNAs whose export is dependent on GANP have a small number of exons, a high mean exon length, long 3’ UTR, and low GC content. Some of these features are shared by influenza virus mRNAs. Additionally, we identified a 45 nucleotide RNA signal from influenza virus HA mRNA that is sufficient to mediate GANP-dependent mRNA export. Thus, we report a role for the TREX-2 complex in nuclear export of influenza mRNAs and identified RNA determinants associated with the TREX-2-dependent mRNA export.

Suggested Citation

  • Prasanna Bhat & Vasilisa Aksenova & Matthew Gazzara & Emily A. Rex & Sadaf Aslam & Christina Haddad & Shengyan Gao & Matthew Esparza & Tolga Cagatay & Kimberly Batten & Sara S. El Zahed & Alexei Arnao, 2023. "Influenza virus mRNAs encode determinants for nuclear export via the cellular TREX-2 complex," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37911-0
    DOI: 10.1038/s41467-023-37911-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37911-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37911-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Vasilisa Aksenova & Alexandra Smith & Hangnoh Lee & Prasanna Bhat & Caroline Esnault & Shane Chen & James Iben & Ross Kaufhold & Ka Chun Yau & Carlos Echeverria & Beatriz Fontoura & Alexei Arnaoutov &, 2020. "Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Bradley P. Clarke & Shengyan Gao & Menghan Mei & Dongqi Xie & Alexia E. Angelos & Ashley Vazhavilla & Pate S. Hill & Tolga Cagatay & Kimberly Batten & Jerry W. Shay & Yihu Xie & Beatriz M. A. Fontoura, 2025. "Structural mechanism of DDX39B regulation by human TREX-2 and a related complex in mRNP remodeling," Nature Communications, Nature, vol. 16(1), pages 1-21, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Bradley P. Clarke & Shengyan Gao & Menghan Mei & Dongqi Xie & Alexia E. Angelos & Ashley Vazhavilla & Pate S. Hill & Tolga Cagatay & Kimberly Batten & Jerry W. Shay & Yihu Xie & Beatriz M. A. Fontoura, 2025. "Structural mechanism of DDX39B regulation by human TREX-2 and a related complex in mRNP remodeling," Nature Communications, Nature, vol. 16(1), pages 1-21, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37911-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.