IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37348-5.html
   My bibliography  Save this article

Identification of tagged glycans with a protein nanopore

Author

Listed:
  • Minmin Li

    (Chinese Academy of Sciences
    East China University of Technology)

  • Yuting Xiong

    (Chinese Academy of Sciences
    East China University of Technology)

  • Yuchen Cao

    (Chinese Academy of Sciences)

  • Chen Zhang

    (Ganjiang Chinese Medicine Innovation Center)

  • Yuting Li

    (Ganjiang Chinese Medicine Innovation Center)

  • Hanwen Ning

    (Zhongnan University of Economics and Law)

  • Fan Liu

    (Chinese Academy of Sciences)

  • Han Zhou

    (Chinese Academy of Sciences
    Ganjiang Chinese Medicine Innovation Center)

  • Xiaonong Li

    (Ganjiang Chinese Medicine Innovation Center)

  • Xianlong Ye

    (Ganjiang Chinese Medicine Innovation Center)

  • Yue Pang

    (Liaoning Normal University)

  • Jiaming Zhang

    (Zhongnan University of Economics and Law)

  • Xinmiao Liang

    (Chinese Academy of Sciences
    Ganjiang Chinese Medicine Innovation Center)

  • Guangyan Qing

    (Chinese Academy of Sciences)

Abstract

Structural complexity of glycans derived from the diversities in composition, linage, configuration, and branching considerably complicates structural analysis. Nanopore-based single-molecule sensing offers the potential to elucidate glycan structure and even sequence glycan. However, the small molecular size and low charge density of glycans have restricted direct nanopore detection of glycan. Here we show that glycan sensing can be achieved using a wild-type aerolysin nanopore by introducing a facile glycan derivatization strategy. The glycan molecule can induce impressive current blockages when moving through the nanopore after being connected with an aromatic group-containing tag (plus a carrier group for the neutral glycan). The obtained nanopore data permit the identification of glycan regio- and stereoisomers, glycans with variable monosaccharide numbers, and distinct branched glycans, either independently or with the use of machine learning methods. The presented nanopore sensing strategy for glycans paves the way towards nanopore glycan profiling and potentially sequencing.

Suggested Citation

  • Minmin Li & Yuting Xiong & Yuchen Cao & Chen Zhang & Yuting Li & Hanwen Ning & Fan Liu & Han Zhou & Xiaonong Li & Xianlong Ye & Yue Pang & Jiaming Zhang & Xinmiao Liang & Guangyan Qing, 2023. "Identification of tagged glycans with a protein nanopore," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37348-5
    DOI: 10.1038/s41467-023-37348-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37348-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37348-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Buddini Iroshika Karawdeniya & Y. M. Nuwan D. Y. Bandara & Jonathan W. Nichols & Robert B. Chevalier & Jason R. Dwyer, 2018. "Surveying silicon nitride nanopores for glycomics and heparin quality assurance," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    2. J. Hofmann & H. S. Hahm & P. H. Seeberger & K. Pagel, 2015. "Identification of carbohydrate anomers using ion mobility–mass spectrometry," Nature, Nature, vol. 526(7572), pages 241-244, October.
    3. Parisa Bayat & Charlotte Rambaud & Bernard Priem & Matthieu Bourderioux & Mélanie Bilong & Salomé Poyer & Manuela Pastoriza-Gallego & Abdelghani Oukhaled & Jérôme Mathé & Régis Daniel, 2022. "Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Chan Cao & Nuria Cirauqui & Maria Jose Marcaida & Elena Buglakova & Alice Duperrex & Aleksandra Radenovic & Matteo Dal Peraro, 2019. "Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    5. Chan Cao & Meng-Yin Li & Nuria Cirauqui & Ya-Qian Wang & Matteo Dal Peraro & He Tian & Yi-Tao Long, 2018. "Mapping the sensing spots of aerolysin for single oligonucleotides analysis," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    6. Felipe Rivas & Osama K. Zahid & Heidi L. Reesink & Bridgette T. Peal & Alan J. Nixon & Paul L. DeAngelis & Aleksander Skardal & Elaheh Rahbar & Adam R. Hall, 2018. "Label-free analysis of physiological hyaluronan size distribution with a solid-state nanopore sensor," Nature Communications, Nature, vol. 9(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Parisa Bayat & Charlotte Rambaud & Bernard Priem & Matthieu Bourderioux & Mélanie Bilong & Salomé Poyer & Manuela Pastoriza-Gallego & Abdelghani Oukhaled & Jérôme Mathé & Régis Daniel, 2022. "Comprehensive structural assignment of glycosaminoglycan oligo- and polysaccharides by protein nanopore," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Xiaoyu Zhou & Zhuofan Wang & Jingjin Fan & Zheng Ouyang, 2023. "High-resolution separation of bioisomers using ion cloud profiling," Nature Communications, Nature, vol. 14(1), pages 1-6, December.
    3. Seu-Na Lee & Hwa-Jin Cho & Hyeongseop Jeong & Bumhan Ryu & Hyuk-Joon Lee & Minsoo Kim & Jejoong Yoo & Jae-Sung Woo & Hyung Ho Lee, 2023. "Cryo-EM structures of human Cx36/GJD2 neuronal gap junction channel," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    4. Fushi Wang & Chunxiao Zhao & Pinlong Zhao & Fanfan Chen & Dan Qiao & Jiandong Feng, 2023. "MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution," Nature Communications, Nature, vol. 14(1), pages 1-8, December.
    5. Gerardo Patiño-Guillén & Jovan Pešović & Marko Panić & Dušanka Savić-Pavićević & Filip Bošković & Ulrich Felix Keyser, 2024. "Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    6. Lauren S. Lastra & Y. M. Nuwan D. Y. Bandara & Michelle Nguyen & Nasim Farajpour & Kevin J. Freedman, 2022. "On the origins of conductive pulse sensing inside a nanopore," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    7. Leïla Bechtella & Jin Chunsheng & Kerstin Fentker & Güney R. Ertürk & Marc Safferthal & Łukasz Polewski & Michael Götze & Simon Y. Graeber & Gaël M. Vos & Weston B. Struwe & Marcus A. Mall & Philipp M, 2024. "Ion mobility-tandem mass spectrometry of mucin-type O-glycans," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37348-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.