IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-33537-w.html
   My bibliography  Save this article

Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP

Author

Listed:
  • Maria A. Schumacher

    (Duke University School of Medicine)

  • Mirka E. Wörmann

    (Humboldt-Universität zu Berlin
    Bundesinstitut für Risikobewertung)

  • Max Henderson

    (Duke University School of Medicine)

  • Raul Salinas

    (Duke University School of Medicine)

  • Andreas Latoscha

    (Humboldt-Universität zu Berlin)

  • Mahmoud M. Al-Bassam

    (University of California, San Diego)

  • Kumar Siddharth Singh

    (Leibniz Universität Hannover)

  • Elaine Barclay

    (John Innes Centre)

  • Katrin Gunka

    (Leibniz Universität Hannover)

  • Natalia Tschowri

    (Leibniz Universität Hannover)

Abstract

Streptomyces are our principal source of antibiotics, which they generate concomitant with a complex developmental transition from vegetative hyphae to spores. c-di-GMP acts as a linchpin in this transition by binding and regulating the key developmental regulators, BldD and WhiG. Here we show that c-di-GMP also binds the glycogen-debranching-enzyme, GlgX, uncovering a direct link between c-di-GMP and glycogen metabolism in bacteria. Further, we show c-di-GMP binding is required for GlgX activity. We describe structures of apo and c-di-GMP-bound GlgX and, strikingly, their comparison shows c-di-GMP induces long-range conformational changes, reorganizing the catalytic pocket to an active state. Glycogen is an important glucose storage compound that enables animals to cope with starvation and stress. Our in vivo studies reveal the important biological role of GlgX in Streptomyces glucose availability control. Overall, we identify a function of c-di-GMP in controlling energy storage metabolism in bacteria, which is widespread in Actinobacteria.

Suggested Citation

  • Maria A. Schumacher & Mirka E. Wörmann & Max Henderson & Raul Salinas & Andreas Latoscha & Mahmoud M. Al-Bassam & Kumar Siddharth Singh & Elaine Barclay & Katrin Gunka & Natalia Tschowri, 2022. "Allosteric regulation of glycogen breakdown by the second messenger cyclic di-GMP," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33537-w
    DOI: 10.1038/s41467-022-33537-w
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-33537-w
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-33537-w?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33537-w. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.