IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-32114-5.html
   My bibliography  Save this article

Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues

Author

Listed:
  • Wonjun Choi

    (Institute for Basic Science
    Korea University)

  • Munkyu Kang

    (Institute for Basic Science
    Korea University)

  • Jin Hee Hong

    (Institute for Basic Science
    Korea University)

  • Ori Katz

    (The Hebrew University of Jerusalem)

  • Byunghak Lee

    (Korea Electrotechnology Research Institute
    B2LAB co., ltd)

  • Guang Hoon Kim

    (Korea Electrotechnology Research Institute)

  • Youngwoon Choi

    (Korea University
    Korea University)

  • Wonshik Choi

    (Institute for Basic Science
    Korea University)

Abstract

Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-μm spatial resolution. Using a bare fibre bundle as thin as 200-μm diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications.

Suggested Citation

  • Wonjun Choi & Munkyu Kang & Jin Hee Hong & Ori Katz & Byunghak Lee & Guang Hoon Kim & Youngwoon Choi & Wonshik Choi, 2022. "Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32114-5
    DOI: 10.1038/s41467-022-32114-5
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-32114-5
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-32114-5?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Tomáš Čižmár & Kishan Dholakia, 2012. "Exploiting multimode waveguides for pure fibre-based imaging," Nature Communications, Nature, vol. 3(1), pages 1-9, January.
    2. Moonseok Kim & Yonghyeon Jo & Jin Hee Hong & Suhyun Kim & Seokchan Yoon & Kyung-Deok Song & Sungsam Kang & Byunghak Lee & Guang Hoon Kim & Hae-Chul Park & Wonshik Choi, 2019. "Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    3. Sungsam Kang & Pilsung Kang & Seungwon Jeong & Yongwoo Kwon & Taeseok D. Yang & Jin Hee Hong & Moonseok Kim & Kyung–Deok Song & Jin Hyoung Park & Jun Ho Lee & Myoung Joon Kim & Ki Hean Kim & Wonshik C, 2017. "High-resolution adaptive optical imaging within thick scattering media using closed-loop accumulation of single scattering," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sanghyeon Park & Yonghyeon Jo & Minsu Kang & Jin Hee Hong & Sangyoon Ko & Suhyun Kim & Sangjun Park & Hae Chul Park & Sang-Hee Shim & Wonshik Choi, 2023. "Label-free adaptive optics single-molecule localization microscopy for whole zebrafish," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    2. Ye-Ryoung Lee & Dong-Young Kim & Yonghyeon Jo & Moonseok Kim & Wonshik Choi, 2023. "Exploiting volumetric wave correlation for enhanced depth imaging in scattering medium," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    3. Yongwoo Kwon & Jin Hee Hong & Sungsam Kang & Hojun Lee & Yonghyeon Jo & Ki Hean Kim & Seokchan Yoon & Wonshik Choi, 2023. "Computational conjugate adaptive optics microscopy for longitudinal through-skull imaging of cortical myelin," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    4. Flavien Bureau & Justine Robin & Arthur Ber & William Lambert & Mathias Fink & Alexandre Aubry, 2023. "Three-dimensional ultrasound matrix imaging," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. Ziwei Li & Wei Zhou & Zhanhong Zhou & Shuqi Zhang & Jianyang Shi & Chao Shen & Junwen Zhang & Nan Chi & Qionghai Dai, 2024. "Self-supervised dynamic learning for long-term high-fidelity image transmission through unstabilized diffusive media," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    6. Noam Badt & Ori Katz, 2022. "Real-time holographic lensless micro-endoscopy through flexible fibers via fiber bundle distal holography," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    7. Sungsam Kang & Yongwoo Kwon & Hojun Lee & Seho Kim & Jin Hee Hong & Seokchan Yoon & Wonshik Choi, 2023. "Tracing multiple scattering trajectories for deep optical imaging in scattering media," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    8. Miroslav Stibůrek & Petra Ondráčková & Tereza Tučková & Sergey Turtaev & Martin Šiler & Tomáš Pikálek & Petr Jákl & André Gomes & Jana Krejčí & Petra Kolbábková & Hana Uhlířová & Tomáš Čižmár, 2023. "110 μm thin endo-microscope for deep-brain in vivo observations of neuronal connectivity, activity and blood flow dynamics," Nature Communications, Nature, vol. 14(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32114-5. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.