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CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome

Author

Listed:
  • Jennifer Jungfleisch

    (Universitat Pompeu Fabra)

  • René Böttcher

    (Universitat Pompeu Fabra)

  • Marc Talló-Parra

    (Universitat Pompeu Fabra)

  • Gemma Pérez-Vilaró

    (Universitat Pompeu Fabra)

  • Andres Merits

    (University of Tartu)

  • Eva Maria Novoa

    (The Barcelona Institute of Science and Technology)

  • Juana Díez

    (Universitat Pompeu Fabra)

Abstract

Ample evidence indicates that codon usage bias regulates gene expression. How viruses, such as the emerging mosquito-borne Chikungunya virus (CHIKV), express their genomes at high levels despite an enrichment in rare codons remains a puzzling question. Using ribosome footprinting, we analyze translational changes that occur upon CHIKV infection. We show that CHIKV infection induces codon-specific reprogramming of the host translation machinery to favor the translation of viral RNA genomes over host mRNAs with an otherwise optimal codon usage. This reprogramming was mostly apparent at the endoplasmic reticulum, where CHIKV RNAs show high ribosome occupancy. Mechanistically, it involves CHIKV-induced overexpression of KIAA1456, an enzyme that modifies the wobble U34 position in the anticodon of tRNAs, which is required for proper decoding of codons that are highly enriched in CHIKV RNAs. Our findings demonstrate an unprecedented interplay of viruses with the host tRNA epitranscriptome to adapt the host translation machinery to viral production.

Suggested Citation

  • Jennifer Jungfleisch & René Böttcher & Marc Talló-Parra & Gemma Pérez-Vilaró & Andres Merits & Eva Maria Novoa & Juana Díez, 2022. "CHIKV infection reprograms codon optimality to favor viral RNA translation by altering the tRNA epitranscriptome," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31835-x
    DOI: 10.1038/s41467-022-31835-x
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    1. Yok Hian Chionh & Megan McBee & I. Ramesh Babu & Fabian Hia & Wenwei Lin & Wei Zhao & Jianshu Cao & Agnieszka Dziergowska & Andrzej Malkiewicz & Thomas J. Begley & Sylvie Alonso & Peter C. Dedon, 2016. "tRNA-mediated codon-biased translation in mycobacterial hypoxic persistence," Nature Communications, Nature, vol. 7(1), pages 1-12, December.
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