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Unmixing for ultra-high-plex fluorescence imaging

Author

Listed:
  • Archibald Enninful

    (Yale University)

  • Alev Baysoy

    (Yale University)

  • Rong Fan

    (Yale University
    Yale School of Medicine
    Yale Cancer Center and Yale Stem Cell Center, Yale School of Medicine
    Human and Translational Immunology Program, Yale School of Medicine)

Abstract

Writing in Nature communications, Seo and collaborators presented PICASSO as a method to achieve 15-color imaging of spatially overlapping proteins using no reference emission spectra in a single staining and imaging round. This accessible tool has the potential to be applied to diverse applications within the spatial biology field without neglecting accuracy.

Suggested Citation

  • Archibald Enninful & Alev Baysoy & Rong Fan, 2022. "Unmixing for ultra-high-plex fluorescence imaging," Nature Communications, Nature, vol. 13(1), pages 1-3, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31110-z
    DOI: 10.1038/s41467-022-31110-z
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    References listed on IDEAS

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    1. Alex M. Valm & Sarah Cohen & Wesley R. Legant & Justin Melunis & Uri Hershberg & Eric Wait & Andrew R. Cohen & Michael W. Davidson & Eric Betzig & Jennifer Lippincott-Schwartz, 2017. "Applying systems-level spectral imaging and analysis to reveal the organelle interactome," Nature, Nature, vol. 546(7656), pages 162-167, June.
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