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Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata

Author

Listed:
  • Yu Wang

    (Northeast Forestry University
    Northeast Forestry University)

  • He Zhang

    (Northeast Forestry University
    Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education)

  • Hyok Chol Ri

    (Northeast Forestry University
    University of Science)

  • Zeyu An

    (Northeast Forestry University)

  • Xin Wang

    (Northeast Forestry University)

  • Jia-Nan Zhou

    (Northeast Forestry University)

  • Dongran Zheng

    (Northeast Forestry University)

  • Hao Wu

    (Northeast Forestry University
    Northeast Forestry University)

  • Pengchao Wang

    (Northeast Forestry University)

  • Jianfei Yang

    (Northeast Forestry University)

  • Ding-Kun Liu

    (Fujian Agriculture and Forestry University)

  • Diyang Zhang

    (Fujian Agriculture and Forestry University)

  • Wen-Chieh Tsai

    (National Cheng Kung University
    National Cheng Kung University)

  • Zheyong Xue

    (Northeast Forestry University
    Northeast Forestry University)

  • Zhichao Xu

    (Northeast Forestry University
    Northeast Forestry University)

  • Peng Zhang

    (Northeast Forestry University)

  • Zhong-Jian Liu

    (Fujian Agriculture and Forestry University)

  • Hailong Shen

    (Northeast Forestry University
    Northeast Forestry University)

  • Yuhua Li

    (Northeast Forestry University
    Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education)

Abstract

Araliaceae species produce various classes of triterpene and triterpenoid saponins, such as the oleanane-type triterpenoids in Aralia species and dammarane-type saponins in Panax, valued for their medicinal properties. The lack of genome sequences of Panax relatives has hindered mechanistic insight into the divergence of triterpene saponins in Araliaceae. Here, we report a chromosome-level genome of Aralia elata with a total length of 1.05 Gb. The loss of 12 exons in the dammarenediol synthase (DDS)-encoding gene in A. elata after divergence from Panax might have caused the lack of dammarane-type saponin production, and a complementation assay shows that overexpression of the PgDDS gene from Panax ginseng in callus of A. elata recovers the accumulation of dammarane-type saponins. Tandem duplication events of triterpene biosynthetic genes are common in the A. elata genome, especially for AeCYP72As, AeCSLMs, and AeUGT73s, which function as tailoring enzymes of oleanane-type saponins and aralosides. More than 13 aralosides are de novo synthesized in Saccharomyces cerevisiae by overexpression of these genes in combination. This study sheds light on the diversity of saponins biosynthetic pathway in Araliaceae and will facilitate heterologous bioproduction of aralosides.

Suggested Citation

  • Yu Wang & He Zhang & Hyok Chol Ri & Zeyu An & Xin Wang & Jia-Nan Zhou & Dongran Zheng & Hao Wu & Pengchao Wang & Jianfei Yang & Ding-Kun Liu & Diyang Zhang & Wen-Chieh Tsai & Zheyong Xue & Zhichao Xu , 2022. "Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29908-y
    DOI: 10.1038/s41467-022-29908-y
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    References listed on IDEAS

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    1. Soo Yeon Chung & Hikaru Seki & Yukiko Fujisawa & Yoshikazu Shimoda & Susumu Hiraga & Yuhta Nomura & Kazuki Saito & Masao Ishimoto & Toshiya Muranaka, 2020. "A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis," Nature Communications, Nature, vol. 11(1), pages 1-11, December.
    2. Yu Wang & He Zhang & Hyok Chol Ri & Zeyu An & Xin Wang & Jia-Nan Zhou & Dongran Zheng & Hao Wu & Pengchao Wang & Jianfei Yang & Ding-Kun Liu & Diyang Zhang & Wen-Chieh Tsai & Zheyong Xue & Zhichao Xu , 2022. "Author Correction: Deletion and tandem duplications of biosynthetic genes drive the diversity of triterpenoids in Aralia elata," Nature Communications, Nature, vol. 13(1), pages 1-2, December.
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    Cited by:

    1. Jiao Yang & Ying Wu & Pan Zhang & Jianxiang Ma & Ying Jun Yao & Yan Lin Ma & Lei Zhang & Yongzhi Yang & Changmin Zhao & Jihua Wu & Xiangwen Fang & Jianquan Liu, 2023. "Multiple independent losses of the biosynthetic pathway for two tropane alkaloids in the Solanaceae family," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    2. Wei Sun & Qinggang Yin & Huihua Wan & Ranran Gao & Chao Xiong & Chong Xie & Xiangxiao Meng & Yaolei Mi & Xiaotong Wang & Caixia Wang & Weiqiang Chen & Ziyan Xie & Zheyong Xue & Hui Yao & Peng Sun & Xu, 2023. "Characterization of the horse chestnut genome reveals the evolution of aescin and aesculin biosynthesis," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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