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A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy

Author

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  • Gangwei Jiang

    (College of Chemistry and Chemical Engineering, Hunan University)

  • Tian-Bing Ren

    (College of Chemistry and Chemical Engineering, Hunan University)

  • Elisa D’Este

    (Max Planck Institute for Medical Research)

  • Mengyi Xiong

    (College of Chemistry and Chemical Engineering, Hunan University)

  • Bin Xiong

    (College of Chemistry and Chemical Engineering, Hunan University)

  • Kai Johnsson

    (Max Planck Institute for Medical Research
    École Polytechnique Fédérale de Lausanne (EPFL))

  • Xiao-Bing Zhang

    (College of Chemistry and Chemical Engineering, Hunan University)

  • Lu Wang

    (Max Planck Institute for Medical Research
    Ministry of Education, School of Pharmacy)

  • Lin Yuan

    (College of Chemistry and Chemical Engineering, Hunan University)

Abstract

The quality and application of super-resolution fluorescence imaging greatly lie in the dyes’ properties, including photostability, brightness, and Stokes shift. Here we report a synergistic strategy to simultaneously improve such properties of regular fluorophores. Introduction of quinoxaline motif with fine-tuned electron density to conventional rhodamines generates new dyes with vibration structure and inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing the brightness and photostability while enlarging Stokes shift. The new fluorophore YL578 exhibits around twofold greater brightness and Stokes shift than its parental fluorophore, Rhodamine B. Importantly, in Stimulated Emission Depletion (STED) microscopy, YL578 derived probe possesses a superior photostability and thus renders threefold more frames than carbopyronine based probes (CPY-Halo and 580CP-Halo), known as photostable fluorophores for STED imaging. Furthermore, the strategy is well generalized to offer a new class of bright and photostable fluorescent probes with long Stokes shift (up to 136 nm) for bioimaging and biosensing.

Suggested Citation

  • Gangwei Jiang & Tian-Bing Ren & Elisa D’Este & Mengyi Xiong & Bin Xiong & Kai Johnsson & Xiao-Bing Zhang & Lu Wang & Lin Yuan, 2022. "A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29547-3
    DOI: 10.1038/s41467-022-29547-3
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    1. Xusan Yang & Zhigang Yang & Zhaoyang Wu & Ying He & Chunyan Shan & Peiyuan Chai & Chenshuo Ma & Mi Tian & Junlin Teng & Dayong Jin & Wei Yan & Pintu Das & Junle Qu & Peng Xi, 2020. "Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
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