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ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death

Author

Listed:
  • Ronny Völz

    (Seoul National University)

  • William Harris

    (Seoul National University)

  • Heribert Hirt

    (King Abdullah University of Science and Technology
    University of Vienna)

  • Yong-Hwan Lee

    (Seoul National University
    Seoul National University
    Seoul National University
    Seoul National University)

Abstract

Sexual plant reproduction depends on the attraction of sperm-cell delivering pollen tubes (PT) by two synergids, followed by their programmed cell death (PCD) in Arabidopsis. Disruption of the mitogen-activated protein kinase 4 (MPK4) by pathogenic effectors activates the resistance protein (R) SUMM2-mediated immunity and cell death. Here we show that synergid preservation and reactive oxygen species (ROS) homeostasis are intimately linked and maintained by MPK4. In mpk4, ROS levels are increased and synergids prematurely undergo PCD before PT-reception. However, ROS scavengers and the disruption of SUMM2, in mpk4, restore ROS homeostasis, synergid maintenance and PT perception, demonstrating that the guardian of MPK4, SUMM2, triggers synergid-PCD. In mpk4/summ2, PTs show a feronia-like overgrowth phenotype. Our results show that immunity-associated PCD and synergid cell death during plant reproduction are regulated by MPK4 underscoring an underlying molecular mechanism for the suppression of plant reproduction during systemic R-mediated immunity.

Suggested Citation

  • Ronny Völz & William Harris & Heribert Hirt & Yong-Hwan Lee, 2022. "ROS homeostasis mediated by MPK4 and SUMM2 determines synergid cell death," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29373-7
    DOI: 10.1038/s41467-022-29373-7
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    1. Satohiro Okuda & Hiroki Tsutsui & Keiko Shiina & Stefanie Sprunck & Hidenori Takeuchi & Ryoko Yui & Ryushiro D. Kasahara & Yuki Hamamura & Akane Mizukami & Daichi Susaki & Nao Kawano & Takashi Sakakib, 2009. "Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells," Nature, Nature, vol. 458(7236), pages 357-361, March.
    2. Yanyan Huang & Chuanchun Yin & Jun Liu & Baomin Feng & Dongdong Ge & Liang Kong & Fausto Andres Ortiz-Morea & Julia Richter & Marie-Theres Hauser & Wen-Ming Wang & Libo Shan & Ping He, 2020. "A trimeric CrRLK1L-LLG1 complex genetically modulates SUMM2-mediated autoimmunity," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    3. Philipp Denninger & Andrea Bleckmann & Andreas Lausser & Frank Vogler & Thomas Ott & David W. Ehrhardt & Wolf B. Frommer & Stefanie Sprunck & Thomas Dresselhaus & Guido Grossmann, 2014. "Male–female communication triggers calcium signatures during fertilization in Arabidopsis," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    4. Qiaohong Duan & Daniel Kita & Eric A. Johnson & Mini Aggarwal & Laura Gates & Hen-Ming Wu & Alice Y. Cheung, 2014. "Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis," Nature Communications, Nature, vol. 5(1), pages 1-10, May.
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