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Myosin VI regulates the spatial organisation of mammalian transcription initiation

Author

Listed:
  • Yukti Hari-Gupta

    (University of Kent
    University College London)

  • Natalia Fili

    (University of Sheffield
    University of Lincoln)

  • Ália dos Santos

    (University of Sheffield)

  • Alexander W. Cook

    (University of Sheffield)

  • Rosemarie E. Gough

    (University of Sheffield)

  • Hannah C. W. Reed

    (University of Kent)

  • Lin Wang

    (Rutherford Appleton Laboratory, Harwell, Didcot)

  • Jesse Aaron

    (HHMI Janelia Research Campus)

  • Tomas Venit

    (New York University Abu Dhabi (NYUAD))

  • Eric Wait

    (HHMI Janelia Research Campus)

  • Andreas Grosse-Berkenbusch

    (Ulm University)

  • J. Christof M. Gebhardt

    (Ulm University)

  • Piergiorgio Percipalle

    (New York University Abu Dhabi (NYUAD)
    Stockholm University)

  • Teng-Leong Chew

    (HHMI Janelia Research Campus)

  • Marisa Martin-Fernandez

    (Rutherford Appleton Laboratory, Harwell, Didcot)

  • Christopher P. Toseland

    (University of Sheffield)

Abstract

During transcription, RNA Polymerase II (RNAPII) is spatially organised within the nucleus into clusters that correlate with transcription activity. While this is a hallmark of genome regulation in mammalian cells, the mechanisms concerning the assembly, organisation and stability remain unknown. Here, we have used combination of single molecule imaging and genomic approaches to explore the role of nuclear myosin VI (MVI) in the nanoscale organisation of RNAPII. We reveal that MVI in the nucleus acts as the molecular anchor that holds RNAPII in high density clusters. Perturbation of MVI leads to the disruption of RNAPII localisation, chromatin organisation and subsequently a decrease in gene expression. Overall, we uncover the fundamental role of MVI in the spatial regulation of gene expression.

Suggested Citation

  • Yukti Hari-Gupta & Natalia Fili & Ália dos Santos & Alexander W. Cook & Rosemarie E. Gough & Hannah C. W. Reed & Lin Wang & Jesse Aaron & Tomas Venit & Eric Wait & Andreas Grosse-Berkenbusch & J. Chri, 2022. "Myosin VI regulates the spatial organisation of mammalian transcription initiation," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28962-w
    DOI: 10.1038/s41467-022-28962-w
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    References listed on IDEAS

    as
    1. Christopher P. Caridi & Carla D’Agostino & Taehyun Ryu & Grzegorz Zapotoczny & Laetitia Delabaere & Xiao Li & Varandt Y. Khodaverdian & Nuno Amaral & Emily Lin & Alesandra R. Rau & Irene Chiolo, 2018. "Nuclear F-actin and myosins drive relocalization of heterochromatic breaks," Nature, Nature, vol. 559(7712), pages 54-60, July.
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    Cited by:

    1. Jie Shi & Kristine Hauschulte & Ivan Mikicic & Srijana Maharjan & Valerie Arz & Tina Strauch & Jan B. Heidelberger & Jonas V. Schaefer & Birgit Dreier & Andreas Plückthun & Petra Beli & Helle D. Ulric, 2023. "Nuclear myosin VI maintains replication fork stability," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Ália dos Santos & Daniel E. Rollins & Yukti Hari-Gupta & Hannah McArthur & Mingxue Du & Sabrina Yong Zi Ru & Kseniia Pidlisna & Ane Stranger & Faeeza Lorgat & Danielle Lambert & Ian Brown & Kevin Howl, 2023. "Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription," Nature Communications, Nature, vol. 14(1), pages 1-24, December.
    3. Matteo Mazzocca & Alessia Loffreda & Emanuele Colombo & Tom Fillot & Daniela Gnani & Paola Falletta & Emanuele Monteleone & Serena Capozi & Edouard Bertrand & Gaelle Legube & Zeno Lavagnino & Carlo Ta, 2023. "Chromatin organization drives the search mechanism of nuclear factors," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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