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Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Author

Listed:
  • Weronika Witkowska McConnell

    (MRC-University of Glasgow, Centre for Virus Research, University of Glasgow
    University of Glasgow)

  • Chris Davis

    (MRC-University of Glasgow, Centre for Virus Research, University of Glasgow)

  • Suleman R. Sabir

    (MRC-University of Glasgow, Centre for Virus Research, University of Glasgow)

  • Alice Garrett

    (University of Glasgow)

  • Amanda Bradley-Stewart

    (Glasgow Royal Infirmary)

  • Pawel Jajesniak

    (University of Glasgow)

  • Julien Reboud

    (University of Glasgow)

  • Gaolian Xu

    (University of Glasgow)

  • Zhugen Yang

    (University of Glasgow)

  • Rory Gunson

    (Glasgow Royal Infirmary)

  • Emma C. Thomson

    (MRC-University of Glasgow, Centre for Virus Research, University of Glasgow)

  • Jonathan M. Cooper

    (University of Glasgow)

Abstract

The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in

Suggested Citation

  • Weronika Witkowska McConnell & Chris Davis & Suleman R. Sabir & Alice Garrett & Amanda Bradley-Stewart & Pawel Jajesniak & Julien Reboud & Gaolian Xu & Zhugen Yang & Rory Gunson & Emma C. Thomson & Jo, 2021. "Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27076-z
    DOI: 10.1038/s41467-021-27076-z
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